ArticlePLoS computational biology2012
Structural insights into the inhibition of actin-capping protein by interactions with phosphatidic acid and phosphatidylinositol (4,5)-bisphosphate.
Article in PLoS computational biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 46 citations in OpenAlex.
- Phosphatidic acid signaling and function in nuclei.Progress in lipid research · 2024Review
- Phosphatidic Acid in Plant Hormonal Signaling: From Target Proteins to Membrane Conformations.International journal of molecular sciences · 2022Review
- Plasma membrane phospholipid signature recruits the plant exocyst complex via the EXO70A1 subunit.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Physiological role of actin regulation in male fertility: Insight into actin capping proteins in spermatogenic cells.Reproductive medicine and biology · 2020Article
- Division of Labor Between Two Actin Nucleators-the Formin FH1 and the ARP2/3 Complex-inFrontiers in plant science · 2020Article
- Computational Modeling of Realistic Cell Membranes.Chemical reviews · 2019Review
- Multiscale Simulations of Biological Membranes: The Challenge To Understand Biological Phenomena in a Living Substance.Chemical reviews · 2019Article
- Molecular mechanism for inhibition of twinfilin by phosphoinositides.The Journal of biological chemistry · 2018Article
- Mechanistic principles underlying regulation of the actin cytoskeleton by phosphoinositides.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Exocyst SEC3 and Phosphoinositides Define Sites of Exocytosis in Pollen Tube Initiation and Growth.Plant physiology · 2016Article
- Plant Phosphoglycerolipids: The Gatekeepers of Vascular Cell Differentiation.Frontiers in plant science · 2016Review
- Capping protein integrates multiple MAMP signalling pathways to modulate actin dynamics during plant innate immunity.Nature communications · 2015Article
- Phospholipase D and phosphatidic acid in plant defence response: from protein-protein and lipid-protein interactions to hormone signalling.Journal of experimental botany · 2015Review
- Heterodimeric capping protein from Arabidopsis is a membrane-associated, actin-binding protein.Plant physiology · 2014Article
- The availability of filament ends modulates actin stochastic dynamics in live plant cells.Molecular biology of the cell · 2014Article
- When fat is not bad: the regulation of actin dynamics by phospholipid signaling molecules.Frontiers in plant science · 2014Review
- Phosphatidic acid, a versatile water-stress signal in roots.Frontiers in plant science · 2013Review
- Arabidopsis capping protein senses cellular phosphatidic acid levels and transduces these into changes in actin cytoskeleton dynamics.Plant signaling & behavior · 2012Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The actin cytoskeleton is a dynamic structure that coordinates numerous fundamental processes in eukaryotic cells. Dozens of actin-binding proteins are known to be involved in the regulation of actin filament organization or turnover and many of these are stimulus-response regulators of phospholipid signaling. One of these proteins is the heterodimeric actin-capping protein (CP) which binds the barbed end of actin filaments with high affinity and inhibits both addition and loss of actin monomers at this end. The ability of CP to bind filaments is regulated by signaling phospholipids, which inhibit the activity of CP; however, the exact mechanism of this regulation and the residues on CP responsible for lipid interactions is not fully resolved. Here, we focus on the interaction of CP with two signaling phospholipids, phosphatidic acid (PA) and phosphatidylinositol (4,5)-bisphosphate (PIP(2)). Using different methods of computational biology such as homology modeling, molecular docking and coarse-grained molecular dynamics, we uncovered specific modes of high affinity interaction between membranes containing PA/phosphatidylcholine (PC) and plant CP, as well as between PIP(2)/PC and animal CP. In particular, we identified differences in the binding of membrane lipids by animal and plant CP, explaining previously published experimental results. Furthermore, we pinpoint the critical importance of the C-terminal part of plant CPα subunit for CP-membrane interactions. We prepared a GST-fusion protein for the C-terminal domain of plant α subunit and verified this hypothesis with lipid-binding assays in vitro.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.