ArticleDevelopment (Cambridge, England)2012
A fast and sensitive alternative for β-galactosidase detection in mouse embryos.
Article in Development (Cambridge, England), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 33 citations in OpenAlex.
- Sustained YAP1 signaling alters the fates of lutein and Müllerian mesenchymal cells in mice.Reproduction (Cambridge, England) · 2025Article
- CCBE1 in Cardiac Development and Disease.Frontiers in genetics · 2022Review
- XRCC3 loss leads to midgestational embryonic lethality in mice.DNA repair · 2021Article
- Disruption of entireeLife · 2021Article
- Analysis of Gene Expression Using lacZ Reporter Mouse Lines.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Identifying vitamin A signaling by visualizing gene and protein activity, and by quantification of vitamin A metabolites.Methods in enzymology · 2020Article
- An Improved Staining Method for Low SignalBio-protocol · 2019Article
- Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos.Journal of visualized experiments : JoVE · 2018Article
- Autocrine lysophosphatidic acid signaling activates β-catenin and promotes lung allograft fibrosis.The Journal of clinical investigation · 2017Article
- Advantages and Limitations of Salmon-Gal/Tetrazolium Salt Histochemistry for the Detection of LacZ Reporter Gene Activity in Murine Epithelial Tissue.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2017Article
- An improved method with high sensitivity and low background in detecting low β-galactosidase expression in mouse embryos.PloS one · 2017Article
- Comments on Methods to Suppress Endogenous β-Galactosidase Activity in Mouse Tissues Expressing the LacZ Reporter Gene.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2016Article
- miR-322/-503 cluster is expressed in the earliest cardiac progenitor cells and drives cardiomyocyte specification.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Changes in expression of C2cd4c in pancreatic endocrine cells during pancreatic development.FEBS letters · 2016Article
- Germ cell specific overactivation of WNT/βcatenin signalling has no effect on folliculogenesis but causes fertility defects due to abnormal foetal development.Scientific reports · 2016Article
- Therapeutic genome editing by combined viral and non-viral delivery of CRISPR system components in vivo.Nature biotechnology · 2016Article
- Overview and assessment of the histochemical methods and reagents for the detection of β-galactosidase activity in transgenic animals.Anatomical science international · 2016Review
- cKit+ cardiac progenitors of neural crest origin.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- C9orf72 ablation in mice does not cause motor neuron degeneration or motor deficits.Annals of neurology · 2015Article
- Extra-embryonic Wnt3 regulates the establishment of the primitive streak in mice.Developmental biology · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
The bacterial lacZ gene is widely used as a reporter in a myriad of mouse transgenic experiments. β-Galactosidase, encoded by lacZ, is usually detected using X-gal in combination with ferric and ferrous ions. This assay produces a blue indole precipitate that is easy to detect visually. Here, we show that Salmon-gal in combination with tetrazolium salts provides a more sensitive and faster staining reaction than the traditional β-galactosidase assay in mouse embryos. Using a combination of Salmon-gal and tetranitroblue tetrazolium, we were able to visualize the activity of β-galactosidase in embryos at stages when the customary X-gal reaction failed to detect staining. Our studies provide an enhanced alternative for β-galactosidase detection in expression and cell fate studies that use lacZ-based transgenic mouse lines.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.