ArticleJournal of the American Heart Association2012
Measurement of reverse cholesterol transport pathways in humans: in vivo rates of free cholesterol efflux, esterification, and excretion.
Article in Journal of the American Heart Association, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 69 citations in OpenAlex.
- Effect of Moderate Beer Intake on the Lipid Composition of Human Red Blood Cell Membranes.Nutrients · 2024Trial
- Systemic availability and metabolism of colonic-derived short-chain fatty acids in healthy subjects: a stable isotope study.The Journal of physiology · 2017Trial
- Applications of the Cholesterol Metabolite, 4β-Hydroxycholesterol, as a Sensitive Endogenous Biomarker for Hepatic CYP3A Activity Evaluated within a PBPK Framework.Pharmaceutics · 2024Article
- High-Density Lipoprotein Metabolism and Function in Cardiovascular Diseases: What about Aging and Diet Effects?Nutrients · 2024Review
- Hypertension Increases Susceptibility to Experimental Malaria in Mice.Function (Oxford, England) · 2024Article
- Oxidative modification of HDL by lipid aldehydes impacts HDL function.Archives of biochemistry and biophysics · 2022Review
- Red Blood Cell Membrane Cholesterol May Be a Key Regulator of Sickle Cell Disease Microvascular Complications.Membranes · 2022Review
- Cholesterol in the Cell Membrane-An Emerging Player in Atherogenesis.International journal of molecular sciences · 2022Review
- Article
- The role of cholesterol in invasion and growth of malaria parasites.Frontiers in cellular and infection microbiology · 2022Review
- Impact of Dietary Lipids on the Reverse Cholesterol Transport: What We Learned from Animal Studies.Nutrients · 2021Review
- Cholesterol transport between red blood cells and lipoproteins contributes to cholesterol metabolism in blood.Journal of lipid research · 2020Article
- Phospholipid Component Defines Pharmacokinetic and Pharmacodynamic Properties of Synthetic High-Density Lipoproteins.The Journal of pharmacology and experimental therapeutics · 2020Article
- HDL flux is higher in patients with nonalcoholic fatty liver disease.American journal of physiology. Endocrinology and metabolism · 2019Article
- Red blood cells membrane micropolarity as a novel diagnostic indicator of type 1 and type 2 diabetes.Analytica chimica acta: X · 2019Article
- Cholesterol Acceptors Regulate the Lipidome of Macrophage Foam Cells.International journal of molecular sciences · 2019Article
- From High-Density Lipoprotein Cholesterol to Measurements of Function: Prospects for the Development of Tests for High-Density Lipoprotein Functionality in Cardiovascular Disease.Arteriosclerosis, thrombosis, and vascular biology · 2018Review
- Glycation Reduces the Stability of ApoAI and Increases HDL Dysfunction in Diet-Controlled Type 2 Diabetes.The Journal of clinical endocrinology and metabolism · 2018Article
- Endogenous Cholesterol Excretion Is Negatively Associated With Carotid Intima-Media Thickness in Humans.Arteriosclerosis, thrombosis, and vascular biology · 2017Article
- A novel approach to measuring macrophage-specific reverse cholesterol transport in vivo in humans.Journal of lipid research · 2017Article
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Authors and funding
14 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundReverse cholesterol transport from peripheral tissues is considered the principal atheroprotective mechanism of high-density lipoprotein, but quantifying reverse cholesterol transport in humans in vivo remains a challenge. We describe here a method for measuring flux of cholesterol though 3 primary components of the reverse cholesterol transport pathway in vivo in humans: tissue free cholesterol (FC) efflux, esterification of FC in plasma, and fecal sterol excretion of plasma-derived FC. METHODS AND
resultsA constant infusion of [2,3-(13)C(2)]-cholesterol was administered to healthy volunteers. Three-compartment SAAM II (Simulation, Analysis, and Modeling software; SAAM Institute, University of Washington, WA) fits were applied to plasma FC, red blood cell FC, and plasma cholesterol ester (13)C-enrichment profiles. Fecal sterol excretion of plasma-derived FC was quantified from fractional recovery of intravenous [2,3-(13)C(2)]-cholesterol in feces over 7 days. We examined the key assumptions of the method and evaluated the optimal clinical protocol and approach to data analysis and modeling. A total of 17 subjects from 2 study sites (n=12 from first site, age 21 to 75 years, 2 women; n=5 from second site, age 18 to 70 years, 2 women) were studied. Tissue FC efflux was 3.79±0.88 mg/kg per hour (mean ± standard deviation), or ≍8 g/d. Red blood cell-derived flux into plasma FC was 3.38±1.10 mg/kg per hour. Esterification of plasma FC was ≍28% of tissue FC efflux (1.10±0.38 mg/kg per hour). Recoveries were 7% and 12% of administered [2,3-(13)C(2)]-cholesterol in fecal bile acids and neutral sterols, respectively.
conclusionsThree components of systemic reverse cholesterol transport can be quantified, allowing dissection of this important function of high-density lipoprotein in vivo. Effects of lipoproteins, genetic mutations, lifestyle changes, and drugs on these components can be assessed in humans. (J Am Heart Assoc. 2012;1:e001826 doi: 10.1161/JAHA.112.001826.).
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