ArticleProceedings of the National Academy of Sciences of the United States of America2012
Functional and structural analysis of the human SLO3 pH- and voltage-gated K+ channel.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 69 citations in OpenAlex.
- Zinc is a key regulator of the sperm-specific KeLife · 2026Article
- Cell Settling, Migration, and Stochastic Cancer Gene Expression Suggest Potassium Membrane Flux May Initiate pH Reversal.Biomolecules · 2025Article
- Review
- Transmembrane determinants of voltage-gating differences between BK (Slo1) and Slo3 channels.Biophysical journal · 2024Article
- pH Homeodynamics and Male Fertility: A Coordinated Regulation of Acid-Based Balance during Sperm Journey to Fertilization.Biomolecules · 2024Review
- Review
- Signaling Roleplay between Ion Channels during Mammalian Sperm Capacitation.Biomedicines · 2023Review
- SLO3: A Conserved Regulator of Sperm Membrane Potential.International journal of molecular sciences · 2023Review
- Pharmacological Evidence Suggests That Slo3 Channel Is the Principal KInternational journal of molecular sciences · 2023Article
- The Role of Sperm Membrane Potential and Ion Channels in Regulating Sperm Function.International journal of molecular sciences · 2023Review
- SLO3 in the fast lane: The latest male contraceptive target with a promising small-molecule inhibitor.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- A selective inhibitor of the sperm-specific potassium channel SLO3 impairs human sperm function.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The allosteric gating mechanism of the MthK channel.National science review · 2022Article
- Homozygous mutation in SLO3 leads to severe asthenoteratozoospermia due to acrosome hypoplasia and mitochondrial sheath malformations.Reproductive biology and endocrinology : RB&E · 2022Article
- Structure and Function of Ion Channels Regulating Sperm Motility-An Overview.International journal of molecular sciences · 2021Review
- Calcium-Activated KHandbook of experimental pharmacology · 2021Article
- Comparative Analysis of Testicular Histology and lncRNA-mRNA Expression Patterns Between Landes Geese (Frontiers in genetics · 2021Article
- Purification and initial characterization of Plasmodium falciparum KMicrobial cell factories · 2020Article
- Cloning and characterization of the rat Slo3 (KBritish journal of pharmacology · 2020Article
- Differences and Similarities: The Richness of Comparative Sperm Physiology.Physiology (Bethesda, Md.) · 2020Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The activation of eukaryotic SLO K(+) channels by intracellular cues, mediated by a cytoplasmic structure called the gating ring, is central to their physiological roles. SLO3 channels are exclusively expressed in mammalian sperm, where variations of intracellular pH are critical to cellular function. Previous studies primarily focused on the mouse SLO3 orthologue and revealed that, in murine sperm, SLO3 mediates a voltage- and alkalization-activated K(+) current essential to male fertility. Here we investigate the activation of the human SLO3 channel by intracellular pH at the functional and structural level. By using electrophysiology in a heterologous system, we show that human SLO3 opens upon intracellular pH increase and that its expression and functional properties are modulated by LRRC52, a testis-specific accessory subunit. We next present the crystal structure of the human SLO3 gating ring. Comparison with the known structures of the corresponding domain from SLO1, a Ca(2+)-activated homologue, suggests that the SLO3 gating ring structure may represent an open state. Together, these results present insights into the function of a protein expected to be critical for human reproduction and provide a framework to study the mechanism of pH gating in SLO3 channels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.