ArticlePlant physiology2013
The mitochondrial folylpolyglutamate synthetase gene is required for nitrogen utilization during early seedling development in arabidopsis.
Article in Plant physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- An Allele of Glutamate Formiminotransferase Triggers 5-Methyl-Tetrahydrofolate-to-MeFox Conversion and Facilitates Folate Biofortification in Maize.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- The serine-glycine-one-carbon metabolic network orchestrates changes in nitrogen and sulfur metabolism and shapes plant development.The Plant cell · 2024Article
- Article
- Foxtail millet [Frontiers in nutrition · 2023Article
- Metabolic network changes during skotomorphogenesis inPlant direct · 2022Article
- Comparative Transcriptome Analysis Reveals Mechanisms of Folate Accumulation in Maize Grains.International journal of molecular sciences · 2022Article
- The 5-formyl-tetrahydrofolate proteome links folates with C/N metabolism and reveals feedback regulation of folate biosynthesis.The Plant cell · 2021Article
- Genetic and physiological regulation of folate in pak choi (Brassica rapa subsp. Chinensis) germplasm.Journal of experimental botany · 2020Article
- Influence of Sulfonamide Contamination Derived from Veterinary Antibiotics on Plant Growth and Development.Antibiotics (Basel, Switzerland) · 2020Article
- Simultaneous extraction and determination of mono-/polyglutamyl folates using high-performance liquid chromatography-tandem mass spectrometry and its applications in starchy crops.Analytical and bioanalytical chemistry · 2019Article
- Photorespiration is complemented by cyclic electron flow and the alternative oxidase pathway to optimize photosynthesis and protect against abiotic stress.Photosynthesis research · 2019Review
- Molecular Mechanisms of Acclimatization to Phosphorus Starvation and Recovery Underlying Full-Length Transcriptome Profiling in Barley (Frontiers in plant science · 2018Article
- The manipulation of gene expression and the biosynthesis of Vitamin C, E and folate in light-and dark-germination of sweet corn seeds.Scientific reports · 2017Article
- Tetrahydrofolate Modulates Floral Transition through Epigenetic Silencing.Plant physiology · 2017Article
- Folates in Plants: Research Advances and Progress in Crop Biofortification.Frontiers in chemistry · 2017Review
- A Predictive Coexpression Network Identifies Novel Genes Controlling the Seed-to-Seedling Phase Transition in Arabidopsis thaliana.Plant physiology · 2016Article
- Genome-wide identification and transcriptional analysis of folate metabolism-related genes in maize kernels.BMC plant biology · 2015Article
- The maize brown midrib4 (bm4) gene encodes a functional folylpolyglutamate synthase.The Plant journal : for cell and molecular biology · 2015Article
- Loss of function of folylpolyglutamate synthetase 1 reduces lignin content and improves cell wall digestibility in Arabidopsis.Biotechnology for biofuels · 2015Article
- Arabidopsis plastidial folylpolyglutamate synthetase is required for seed reserve accumulation and seedling establishment in darkness.PloS one · 2014Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
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Abstract
Investigations into the biochemical processes and regulatory mechanisms of nitrogen (N) utilization can aid in understanding how N is used efficiently in plants. This report describes a deficiency in N utilization in an Arabidopsis (Arabidopsis thaliana) transfer DNA insertion mutant of the mitochondrial folylpolyglutamate synthetase gene DFC, which catalyzes the conjugation of glutamate residues to the tetrahydrofolate during folate synthesis. The mutant seedlings displayed several metabolic changes that are typical of plant responses to low-N stress, including increased levels of starch and anthocyanin synthesis as well as decreased levels of soluble protein and free amino acid, as compared with those in wild-type seedlings when external N was sufficient. More striking changes were observed when dfc seedlings were grown under N-limited conditions, including shorter primary roots, fewer lateral roots, higher levels of glycine and carbon-N ratios, and lower N content than those in wild-type seedlings. Gene expression studies in mutant seedlings revealed altered transcript levels of several genes involved in folate biosynthesis and N metabolism. The biochemical and metabolic changes also suggested that N assimilation is drastically perturbed due to a loss of DFC function. The observation that elevated CO(2) partly rescued the dfc phenotypes suggests that the alterations in N metabolism in dfc may be mainly due to a defect in photorespiration. These results indicate that DFC is required for N utilization in Arabidopsis and provide new insight into a potential interaction between folate and N metabolism.
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