ArticleThe Journal of clinical investigation1990
Ultrastructural localization of cytochrome b in the membranes of resting and phagocytosing human granulocytes.
Article in The Journal of clinical investigation, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 141 citations in OpenAlex.
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- The Relationship of NADPH Oxidases and Heme Peroxidases: Fallin' in and Out.Frontiers in immunology · 2019Review
- Blastomyces dermatitidis serine protease dipeptidyl peptidase IVA (DppIVA) cleaves ELRCellular microbiology · 2017Article
- Neutrophils to the ROScue: Mechanisms of NADPH Oxidase Activation and Bacterial Resistance.Frontiers in cellular and infection microbiology · 2017Review
- Activation of neuronal NMDA receptors induces superoxide-mediated oxidative stress in neighboring neurons and astrocytes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Targeting microglia-mediated neurotoxicity: the potential of NOX2 inhibitors.Cellular and molecular life sciences : CMLS · 2012Review
- Redox regulation of endothelial canonical transient receptor potential channels.Antioxidants & redox signaling · 2011Review
- Aged garlic extract scavenges superoxide radicals.Plant foods for human nutrition (Dordrecht, Netherlands) · 2011Article
- Localization of NADPH oxidase in sympathetic and sensory ganglion neurons and perivascular nerve fibers.Autonomic neuroscience : basic & clinical · 2009Article
- Macrophage NADPH oxidase flavocytochrome B localizes to the plasma membrane and Rab11-positive recycling endosomes.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Reactive oxygen species in phagocytic leukocytes.Histochemistry and cell biology · 2008Review
- Molecular evolution of the reactive oxygen-generating NADPH oxidase (Nox/Duox) family of enzymes.BMC evolutionary biology · 2007Article
- A regulated adaptor function of p40phox: distinct p67phox membrane targeting by p40phox and by p47phox.Molecular biology of the cell · 2007Article
- Involvement of Rac1 in activation of multicomponent Nox1- and Nox3-based NADPH oxidases.Molecular and cellular biology · 2006Article
- JAM-C is a component of desmosomes and a ligand for CD11b/CD18-mediated neutrophil transepithelial migration.Molecular biology of the cell · 2004Article
- Inhibition of the neutrophil NADPH oxidase by adenosine is associated with increased movement of flavocytochrome b between subcellular fractions.Inflammation · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Affinity-purified rabbit anti-neutrophil cytochrome b light or heavy chain antibodies were used to immunocytochemically and biochemically localize cytochrome b in neutrophils and eosinophils. The antibodies were monospecific, recognizing polypeptides of 91 and 22 kD, respectively, on Western blots of whole neutrophil extracts. The antibodies were used in Western blot analysis of subcellular fractions of purified neutrophils to confirm that the distribution of cytochrome b spectral absorbance matched that of the two subunits. Thin sections of cryofixed, molecular distillation-dried granulocytes were labeled with the anti-cytochrome b antibodies, followed by incubation with biotin-conjugated secondary antibody, and final labeling with streptavidin-conjugated colloidal gold. Electron microscopy revealed that the cytochrome b light and heavy chains were localized primarily (80%) to 0.1-0.2-micron round or elliptical granule-like structures in neutrophils and 0.4-0.5-micron granules in eosinophils. Approximately 20% of the cytochrome b was localized to the surface, confirming the subcellular fractionation studies. Double staining experiments on the neutrophils, using polyclonal rabbit anti-lactoferrin antibody, indicated that the cytochrome-bearing structures also contained lactoferrin and thus were specific granules. When the analysis was performed on neutrophils that had phagocytosed Staphylococcus aureus, cytochrome b was found in the phagosomal membrane adjoining the bacterial cell wall.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.