ArticleCellular microbiology2013
Hepatoma polarization limits CD81 and hepatitis C virus dynamics.
Article in Cellular microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 40 citations in OpenAlex.
- CD82 and Gangliosides Tune CD81 Membrane Behavior.International journal of molecular sciences · 2021Article
- Bacterial flagellin promotes viral entry via an NF-kB and Toll Like Receptor 5 dependent pathway.Scientific reports · 2019Article
- Tetraspanin Tspan9 regulates platelet collagen receptor GPVI lateral diffusion and activation.Platelets · 2017Article
- Hepatitis C Virus-Genotype 3: Update on Current and Emergent Therapeutic Interventions.Current infectious disease reports · 2017Review
- TNF superfamily members promote hepatitis C virus entry via an NF-κB and myosin light chain kinase dependent pathway.The Journal of general virology · 2017Article
- PI3K/SHIP2/PTEN pathway in cell polarity and hepatitis C virus pathogenesis.World journal of hepatology · 2017Review
- Roles of lipoprotein receptors in the entry of hepatitis C virus.World journal of hepatology · 2015Review
- Structural and functional hepatocyte polarity and liver disease.Journal of hepatology · 2015Review
- Quantitative Proteomics Identifies Serum Response Factor Binding Protein 1 as a Host Factor for Hepatitis C Virus Entry.Cell reports · 2015Article
- Article
- Hepatitis C virus life cycle and lipid metabolism.Biology · 2014Review
- The extracellular δ-domain is essential for the formation of CD81 tetraspanin webs.Biophysical journal · 2014Article
- Review
- Activated macrophages promote hepatitis C virus entry in a tumor necrosis factor-dependent manner.Hepatology (Baltimore, Md.) · 2014Article
- Article
- Review
- CD81-receptor associations--impact for hepatitis C virus entry and antiviral therapies.Viruses · 2014Review
- CD81 and hepatitis C virus (HCV) infection.Viruses · 2014Review
- IFITMs restrict the replication of multiple pathogenic viruses.Journal of molecular biology · 2013Review
- EWI-2wint promotes CD81 clustering that abrogates Hepatitis C Virus entry.Cellular microbiology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 3 institutions in 3 countries.
Funding
Abstract
Many viruses target the polarized epithelial apex during host invasion. In contrast, hepatitis C virus (HCV) engages receptors at the basal surface of hepatocytes in the polarized liver parenchyma. Hepatocyte polarization limits HCV entry by undefined mechanism(s). Given the recent reports highlighting a role for receptor mobility in pathogen entry, we studied the effect(s) of hepatocyte polarization on viral receptor and HCV pseudoparticle (HCVpp) dynamics using real-time fluorescence recovery after photobleaching and single particle tracking. Hepatoma polarization reduced CD81 and HCVpp dynamics at the basal membrane. Since cell polarization is accompanied by changes in the actin cytoskeleton and CD81 links to actin via its C-terminus, we studied the dynamics of a mutant CD81 lacking a C-terminal tail (CD81(ΔC)) and its effect(s) on HCVpp mobility and infection. CD81(ΔC) showed an increased frequency of confined trajectories and a reduction of Brownian diffusing molecules compared to wild-type protein in non-polarized cells. However, these changes were notobserved in polarized cells. HCVpp showed a significant reduction in Brownian diffusion and infection of CD81(ΔC) expressing non-polarized cells. In summary, these data highlight the dynamic nature of CD81 and demonstrate a role for CD81 lateral diffusion to regulate HCV infection in a polarization-dependent manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.