ArticleBMC plant biology2012
KNOX1 is expressed and epigenetically regulated during in vitro conditions in Agave spp.
Article in BMC plant biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 40 citations in OpenAlex.
- Somaclonal Variation-Advantage or Disadvantage in Micropropagation of the Medicinal Plants.International journal of molecular sciences · 2023Review
- Tissue culture-induced DNA methylation in crop plants: a review.Molecular biology reports · 2021Review
- A peak in global DNA methylation is a key step to initiate the somatic embryogenesis of coconut palm (Cocos nucifera L).Plant cell reports · 2020Article
- The Methylation Patterns and Transcriptional Responses to Chilling Stress at the Seedling Stage in Rice.International journal of molecular sciences · 2019Article
- Retroelements and DNA Methylation Could Contribute to Diversity of 5S rDNA in Agave L.Journal of molecular evolution · 2018Article
- Assessment of genetic and epigenetic changes in virus-free garlic (Allium sativum L.) plants obtained by meristem culture followed by in vitro propagation.Plant cell reports · 2016Article
- The role of chromatin modifications in somatic embryogenesis in plants.Frontiers in plant science · 2015Review
- Article
- Somatic Embryogenesis: Identified Factors that Lead to Embryogenic Repression. A Case of Species of the Same Genus.PloS one · 2015Article
- Epi-fingerprinting and epi-interventions for improved crop production and food quality.Frontiers in plant science · 2015Review
- Biotic interactions in the rhizosphere: a diverse cooperative enterprise for plant productivity.Plant physiology · 2014Review
- An efficient immunodetection method for histone modifications in plants.Plant methods · 2013Article
- Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe micropropagation is a powerful tool to scale up plants of economical and agronomical importance, enhancing crop productivity. However, a small but growing body of evidence suggests that epigenetic mechanisms, such as DNA methylation and histone modifications, can be affected under the in vitro conditions characteristic of micropropagation. Here, we tested whether the adaptation to different in vitro systems (Magenta boxes and Bioreactors) modified epigenetically different clones of Agave fourcroydes and A. angustifolia. Furthermore, we assessed whether these epigenetic changes affect the regulatory expression of KNOTTED1-like HOMEOBOX (KNOX) transcription factors.
resultsTo gain a better understanding of epigenetic changes during in vitro and ex vitro conditions in Agave fourcroydes and A. angustifolia, we analyzed global DNA methylation, as well as different histone modification marks, in two different systems: semisolid in Magenta boxes (M) and temporary immersion in modular Bioreactors (B). No significant difference was found in DNA methylation in A. fourcroydes grown in either M or B. However, when A. fourcroydes was compared with A. angustifolia, there was a two-fold difference in DNA methylation between the species, independent of the in vitro system used. Furthermore, we detected an absence or a low amount of the repressive mark H3K9me2 in ex vitro conditions in plants that were cultured earlier either in M or B. Moreover, the expression of AtqKNOX1 and AtqKNOX2, on A. fourcroydes and A. angustifolia clones, is affected during in vitro conditions. Therefore, we used Chromatin ImmunoPrecipitation (ChIP) to know whether these genes were epigenetically regulated. In the case of AtqKNOX1, the H3K4me3 and H3K9me2 were affected during in vitro conditions in comparison with AtqKNOX2.
conclusionsAgave clones plants with higher DNA methylation during in vitro conditions were better adapted to ex vitro conditions. In addition, A. fourcroydes and A. angustifolia clones displayed differential expression of the KNOX1 gene during in vitro conditions, which is epigenetically regulated by the H3K4me3 and H3K9me2 marks. The finding of an epigenetic regulation in key developmental genes will make it important in future studies to identify factors that help to find climate-resistant micropropagated plants.
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