ArticleAmerican journal of physiology. Heart and circulatory physiology2013
An angiogenesis model for investigating multicellular interactions across intact microvascular networks.
Article in American journal of physiology. Heart and circulatory physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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Who cites it
38 citing papers in PubMed, 63 citations in OpenAlex.
- A Novel in vivo Rat Mesentery Model for Studying Tumor Spheroid-Induced Microvascular Remodeling.Journal of vascular research · 2025Article
- Time-Lapse Observation of Cell Dynamics During Angiogenesis Using the Rat Mesentery Culture Model.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Effect of Semaglutide and Empagliflozin on Pulmonary Structure and Proteomics in Obese Mice.Diabetes, metabolic syndrome and obesity : targets and therapy · 2024Article
- Variations in mechanical stiffness alter microvascular sprouting and stability in a PEG hydrogel model of idiopathic pulmonary fibrosis.Microcirculation (New York, N.Y. : 1994) · 2023Article
- Lymphatic/blood vessel plasticity: motivation for a future research area based on present and past observations.American journal of physiology. Heart and circulatory physiology · 2023Review
- Angiogenic Microvascular Wall Shear Stress Patterns Revealed Through Three-dimensional Red Blood Cell Resolved Modeling.Function (Oxford, England) · 2023Article
- A Novel ex vivo Method for Investigating Vascularization of Transplanted Islets.Journal of vascular research · 2022Article
- The Microvascular-Lymphatic Interface and Tissue Homeostasis: Critical Questions That Challenge Current Understanding.Journal of vascular research · 2022Review
- A Challenge for Engineering Biomimetic Microvascular Models: How do we Incorporate the Physiology?Frontiers in bioengineering and biotechnology · 2022Review
- Stromal Vascular Fraction Reverses the Age-Related Impairment in Revascularization following Injury.Journal of vascular research · 2022Article
- Bioprinting on Live Tissue for Investigating Cancer Cell Dynamics.Tissue engineering. Part A · 2021Article
- Potential Biomarkers to Predict Acute Ischemic Stroke in Type 2 Diabetes.Frontiers in molecular biosciences · 2021Article
- The maintenance of adult peripheral adult nerve and microvascular networks in the rat mesentery culture model.Journal of neuroscience methods · 2020Article
- Blocking endothelial apoptosis revascularizes the retina in a model of ischemic retinopathy.The Journal of clinical investigation · 2020Article
- A novel tissue culture model for evaluating the effect of aging on stem cell fate in adult microvascular networks.GeroScience · 2020Article
- Lymphatic-to-blood vessel transition in adult microvascular networks: A discovery made possible by a top-down approach to biomimetic model development.Microcirculation (New York, N.Y. : 1994) · 2020Article
- Application of microscale culture technologies for studying lymphatic vessel biology.Microcirculation (New York, N.Y. : 1994) · 2019Review
- Bioreactor System to Perfuse Mesentery Microvascular Networks and Study Flow Effects During Angiogenesis.Tissue engineering. Part C, Methods · 2019Article
- Proteomic profiling of plasma biomarkers in acute ischemic stroke due to large vessel occlusion.Journal of translational medicine · 2019Observational
- Lymphatic Vessel Network Structure and Physiology.Comprehensive Physiology · 2018Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Developing therapies aimed at manipulating microvascular remodeling requires a better understanding of angiogenesis and how angiogenesis relates to other network remodeling processes, such as lymphangiogenesis and neurogenesis. The objective of this study was to develop an angiogenesis model that enables probing of multicellular and multisystem interactions at the molecular level across an intact adult microvascular network. Adult male Wistar rat mesenteric windows were aseptically harvested and cultured in serum-free minimum essential media. Viability/cytotoxicity analysis revealed that cells remain alive for at least 7 days. Immunohistochemical labeling at 3 days for platelet endothelial cell adhesion molecule (PECAM), neuron-glial antigen 2 (NG2), lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1), and class III β-tubulin identified endothelial cells, pericytes, lymphatics, and nerves, respectively. Media supplemented with bFGF or VEGF induced an increase in endothelial cell sprouting off existing vessels. Endothelial cell sprouting in both growth factor groups was inhibited by targeting pericytes with NG2 functional blocking antibody. VEGF caused an increase in the number of lymphatic/blood endothelial cell connections compared with media alone or bFGF groups. Finally, the comparison of the same network before and after angiogenesis stimulated by the supplement of media with 20% serum identified the ability of disconnected endothelial segments to reconnect to nearby vessels. The results establish a novel in situ angiogenesis model for investigating the location of capillary sprouting within an intact network, the role of pericytes, lymphatic/blood endothelial cell interactions, and the fate of specific endothelial cell segments. The rat mesentery culture system offers a unique tool for understanding the complex dynamics associated with angiogenesis in an intact adult tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.