ArticleIn vitro cellular & developmental biology : journal of the Tissue Culture Association1990
Myogenesis and histogenesis of skeletal muscle on flexible membranes in vitro.
Article in In vitro cellular & developmental biology : journal of the Tissue Culture Association, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 80 citations in OpenAlex.
- Advances in treatment and repair technologies for exercise-induced skeletal muscle injuries.Frontiers in sports and active living · 2025Review
- Review
- Available In Vitro Models for Human Satellite Cells from Skeletal Muscle.International journal of molecular sciences · 2021Review
- Tissue-Engineered Skeletal Muscle Models to Study Muscle Function, Plasticity, and Disease.Frontiers in physiology · 2021Review
- Engineered skeletal muscles for disease modeling and drug discovery.Biomaterials · 2019Review
- Culturing C2C12 myotubes on micromolded gelatin hydrogels accelerates myotube maturation.Skeletal muscle · 2019Article
- Engineering Biomimetic Materials for Skeletal Muscle Repair and Regeneration.Advanced healthcare materials · 2019Review
- In Vitro Tissue-Engineered Skeletal Muscle Models for Studying Muscle Physiology and Disease.Advanced healthcare materials · 2018Review
- Striated muscle function, regeneration, and repair.Cellular and molecular life sciences : CMLS · 2016Review
- Design, evaluation, and application of engineered skeletal muscle.Methods (San Diego, Calif.) · 2016Review
- Synergizing Engineering and Biology to Treat and Model Skeletal Muscle Injury and Disease.Annual review of biomedical engineering · 2015Review
- Skeletal muscle tissue engineering: methods to form skeletal myotubes and their applications.Tissue engineering. Part B, Reviews · 2014Review
- Self-organization and the self-assembling process in tissue engineering.Annual review of biomedical engineering · 2013Review
- Engineering skeletal muscle tissue--new perspectives in vitro and in vivo.Journal of cellular and molecular medicine · 2010Review
- Chromatin organization measured by AluI restriction enzyme changes with malignancy and is regulated by the extracellular matrix and the cytoskeleton.The American journal of pathology · 2005Article
- A simplified method for tissue engineering skeletal muscle organoids in vitro.In vitro cellular & developmental biology. Animal · 1997Article
- Skeletal muscle satellite cells cultured in simulated microgravity.In vitro cellular & developmental biology. Animal · 1997Article
- Muscle-specific splicing enhancers regulate inclusion of the cardiac troponin T alternative exon in embryonic skeletal muscle.Molecular and cellular biology · 1996Article
- The cardiac troponin T alternative exon contains a novel purine-rich positive splicing element.Molecular and cellular biology · 1993Article
- Long-term maintenance of primary myogenic cultures on a reconstituted basement membrane.In vitro cellular & developmental biology : journal of the Tissue Culture Association · 1990Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Primary muscle cell cultures consisting of single myocytes and fibroblasts are grown on flexible, optically clear biomembranes. Muscle cell growth, fusion and terminal differentiation are normal. A most effective membrane for these cultures is commercially available Saran Wrap. Muscle cultures on Saran will, once differentiated, contract vigorously and will deform the Saran which is pinned to a Sylgard base. At first, the muscle forms a two-dimensional network which ultimately detaches from the Saran membrane allowing an undergrowth of fibroblasts so that these connective tissue cells completely surround groups of muscle fibers. A three-dimensional network is thus formed, held in place through durable adhesions to stainless steel pins. This three-dimensional, highly contractile network is seen to consist of all three connective tissue compartments seen in vivo, the endomysium, perimysium and epimysium. Finally, this muscle shows advanced levels of maturation in that neonatal and adult isoforms of myosin heavy chain are detected together with high levels of myosin fast light chain 3.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.