ArticlePlant physiology2013
Tnt1 retrotransposon mutagenesis: a tool for soybean functional genomics.
Article in Plant physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 74 citations in OpenAlex.
- TAIL-PCR for the Recovery of Tnt1 Flanking Sequences in Chickpea: A Tool for Functional Genomics Studies.Methods in molecular biology (Clifton, N.J.) · 2026Article
- The GmPRL1b-GmST2-GmAOC3/4 Module Confers Salt Tolerance and Botrytis cinerea Resistance by Inducing Jasmonic Acid Biosynthesis in Soybean.Plant biotechnology journal · 2025Article
- Cotton transposon-related variome reveals roles of transposon-related variations in modern cotton cultivation.Journal of advanced research · 2025Article
- A strategy for identification and characterization of genic mutations using a temperature-sensitive chlorotic soybean mutant as an example.Plant direct · 2024Article
- Plant Functional Genomics Based on High-Throughput CRISPR Library Knockout Screening: A Perspective.Advanced genetics (Hoboken, N.J.) · 2024Article
- Identification and characterization of a temperature sensitive chlorotic soybean mutant.bioRxiv : the preprint server for biology · 2024Article
- H2O2-dependent oxidation of the transcription factor GmNTL1 promotes salt tolerance in soybean.The Plant cell · 2023Article
- Review
- Reverse genetic approaches for breeding nutrient-rich and climate-resilient cereal and food legume crops.Heredity · 2022Article
- Genetic dissection of the soybean dwarf mutantFrontiers in plant science · 2022Article
- Tracing Mobile DNAs: From Molecular to Population Scales.Frontiers in plant science · 2022Review
- Expanding the Benefits ofFrontiers in plant science · 2021Article
- Development ofPlant direct · 2021Article
- Insertional mutagenesis of Brachypodium distachyon using the Tnt1 retrotransposable element.The Plant journal : for cell and molecular biology · 2020Article
- Generation of a multiplex mutagenesis population via pooled CRISPR-Cas9 in soya bean.Plant biotechnology journal · 2020Article
- The Impacts of Domestication and Agricultural Practices on Legume Nutrient Acquisition Through Symbiosis With Rhizobia and Arbuscular Mycorrhizal Fungi.Frontiers in genetics · 2020Review
- Identification of Transposable Elements in Conifer and Their Potential Application in Breeding.Evolutionary bioinformatics online · 2020Article
- A GmSIN1/GmNCED3s/GmRbohBs Feed-Forward Loop Acts as a Signal Amplifier That Regulates Root Growth in Soybean Exposed to Salt Stress.The Plant cell · 2019Article
- Agrobacterium-mediated Tnt1 mutagenesis of moss protonemal filaments and generation of stable mutants with impaired gametophyte.Molecular genetics and genomics : MGG · 2019Article
- Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insertional mutagenesis is a powerful tool for determining gene function in both model and crop plant species. Tnt1, the transposable element of tobacco (Nicotiana tabacum) cell type 1, is a retrotransposon that replicates via an RNA copy that is reverse transcribed and integrated elsewhere in the plant genome. Based on studies in a variety of plants, Tnt1 appears to be inactive in normal plant tissue but can be reactivated by tissue culture. Our goal was to evaluate the utility of the Tnt1 retrotransposon as a mutagenesis strategy in soybean (Glycine max). Experiments showed that the Tnt1 element was stably transformed into soybean plants by Agrobacterium tumefaciens-mediated transformation. Twenty-seven independent transgenic lines carrying Tnt1 insertions were generated. Southern-blot analysis revealed that the copy number of transposed Tnt1 elements ranged from four to 19 insertions, with an average of approximately eight copies per line. These insertions showed Mendelian segregation and did not transpose under normal growth conditions. Analysis of 99 Tnt1 flanking sequences revealed insertions into 62 (62%) annotated genes, indicating that the element preferentially inserts into protein-coding regions. Tnt1 insertions were found in all 20 soybean chromosomes, indicating that Tnt1 transposed throughout the soybean genome. Furthermore, fluorescence in situ hybridization experiments validated that Tnt1 inserted into multiple chromosomes. Passage of transgenic lines through two different tissue culture treatments resulted in Tnt1 transposition, significantly increasing the number of insertions per line. Thus, our data demonstrate the Tnt1 retrotransposon to be a powerful system that can be used for effective large-scale insertional mutagenesis in soybean.
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