ArticleApplied and environmental microbiology2013
Molecular control of sucrose utilization in Escherichia coli W, an efficient sucrose-utilizing strain.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed, 107 citations in OpenAlex.
- Performance comparison of downstream unit operations and process chains for the purification of amino acids for food, feed and pharma applications.Biotechnology reports (Amsterdam, Netherlands) · 2026Review
- CRISPR-Mediated Metabolic Engineering ofMicroorganisms · 2026Article
- Engineering a Robust Escherichia coli W Platform for Scalable Production of Flavonoid-O-Glucosides.Microbial biotechnology · 2025Article
- Decoupling sucrose utilization from oxygen-responsive regulation for high-efficiency L-lactic acid production in Escherichia coli.Biotechnology for biofuels and bioproducts · 2025Article
- Development of Sucrose-UtilizingMetabolites · 2025Article
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- Synthetic redesign of Escherichia coli W for faster metabolism of sugarcane molasses.Microbial cell factories · 2024Article
- Sucrose as an electron source for cofactor regeneration in recombinant Escherichia coli expressing invertase and a Baeyer Villiger monooxygenase.Microbial cell factories · 2024Article
- Glucose-fed microbiota alters C. elegans intestinal epithelium and increases susceptibility to multiple bacterial pathogens.Scientific reports · 2024Article
- The Scr and Csc pathways for sucrose utilization co-exist inFrontiers in microbiology · 2024Article
- Engineered Sucrose Metabolism Improves the Smut Disease Suppression Potency of Pseudomonas sp. ST4.Applied and environmental microbiology · 2023Article
- Predicting partner fitness based on spatial structuring in a light-driven microbial community.PLoS computational biology · 2023Article
- Cyanobacteria as cell factories for the photosynthetic production of sucrose.Frontiers in microbiology · 2023Review
- A miniaturized bionic ocean-battery mimicking the structure of marine microbial ecosystems.Nature communications · 2022Article
- Biosynthesis of Poly-(3-hydroxybutyrate) under the Control of an Anaerobically Induced Promoter by RecombinantMolecules (Basel, Switzerland) · 2022Article
- A Review on EnhancingFrontiers in bioengineering and biotechnology · 2022Review
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- Construction and analysis of an artificial consortium based on the fast-growing cyanobacteriumBiotechnology for biofuels · 2020Article
- Engineering sucrose metabolism in Pseudomonas putida highlights the importance of porins.Microbial biotechnology · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sucrose is an industrially important carbon source for microbial fermentation. Sucrose utilization in Escherichia coli, however, is poorly understood, and most industrial strains cannot utilize sucrose. The roles of the chromosomally encoded sucrose catabolism (csc) genes in E. coli W were examined by knockout and overexpression experiments. At low sucrose concentrations, the csc genes are repressed and cells cannot grow. Removal of either the repressor protein (cscR) or the fructokinase (cscK) gene facilitated derepression. Furthermore, combinatorial knockout of cscR and cscK conferred an improved growth rate on low sucrose. The invertase (cscA) and sucrose transporter (cscB) genes are essential for sucrose catabolism in E. coli W, demonstrating that no other genes can provide sucrose transport or inversion activities. However, cscK is not essential for sucrose utilization. Fructose is excreted into the medium by the cscK-knockout strain in the presence of high sucrose, whereas at low sucrose (when carbon availability is limiting), fructose is utilized by the cell. Overexpression of cscA, cscAK, or cscAB could complement the WΔcscRKAB knockout mutant or confer growth on a K-12 strain which could not naturally utilize sucrose. However, phenotypic stability and relatively good growth rates were observed in the K-12 strain only when overexpressing cscAB, and full growth rate complementation in WΔcscRKAB also required cscAB. Our understanding of sucrose utilization can be used to improve E. coli W and engineer sucrose utilization in strains which do not naturally utilize sucrose, allowing substitution of sucrose for other, less desirable carbon sources in industrial fermentations.
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