ArticleApplied and environmental microbiology2013
Elimination of manganese(II,III) oxidation in Pseudomonas putida GB-1 by a double knockout of two putative multicopper oxidase genes.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 120 citations in OpenAlex.
- Multi-omic landscape of Mn(Ⅱ) oxidation in Achromobacter pulmonis ss21: from multicopper oxidase to metabolic support electron transfer.World journal of microbiology & biotechnology · 2026Article
- DistinguishingmSystems · 2026Article
- Different Ferromanganese Concretion Morphologies Host Distinct Microbial Communities and Metal Accumulation Patterns in the Baltic Sea.Environmental microbiology · 2026Article
- Enhanced Manganese Oxidation at the Biofilm-Fluid Interface Drives Pore-Scale Patterns in Mineral Precipitation.Environmental science & technology · 2026Article
- Effects of Organic Substrate Concentrations on Biological Mn Oxidation and Reduction under Aerobic Conditions.Microbes and environments · 2026Article
- Article
- Population-level control of two manganese oxidases expands the niche for bacterial manganese biomineralization.NPJ biofilms and microbiomes · 2025Article
- Isolation, characterization, and genetic manipulation of cold-tolerant, manganese-oxidizingApplied and environmental microbiology · 2024Article
- Advances in Research on Bacterial Oxidation of Mn(II): A Visualized Bibliometric Analysis Based on CiteSpace.Microorganisms · 2024Review
- Putative novel hydrogen- and iron-oxidizing sheath-producing Zetaproteobacteria thrive at the Fåvne deep-sea hydrothermal vent field.mSystems · 2023Article
- Biologically Assisted One-Step Synthesis of Electrode Materials for Li-Ion Batteries.Microorganisms · 2023Article
- Novel and unusual genes for nitrogen and metal cycling in Planctomycetota- and KSB1-affiliated metagenome-assembled genomes reconstructed from a marine subsea tunnel.FEMS microbiology letters · 2023Article
- Cyclic di-GMP Signaling Links Biofilm Formation and Mn(II) Oxidation in Pseudomonas resinovorans.mBio · 2022Article
- Mineralogical and Genomic Constraints on the Origin of Microbial Mn Oxide Formation in Complexed Microbial Community at the Terrestrial Hot Spring.Life (Basel, Switzerland) · 2022Article
- A soil-borne Mn(II)-oxidizing bacterium of Providencia sp. exploits a strategy of superoxide production coupled to hydrogen peroxide consumption to generate Mn oxides.Archives of microbiology · 2022Article
- Manganese oxidation counteracts the deleterious effect of low temperatures on biofilm formation inFrontiers in molecular biosciences · 2022Article
- Oligo-heterotrophic Activity ofApplied and environmental microbiology · 2021Article
- Article
- Genome analysis of Pseudomonas sp. OF001 and Rubrivivax sp. A210 suggests multicopper oxidases catalyze manganese oxidation required for cylindrospermopsin transformation.BMC genomics · 2021Article
- Manganese Stress Adaptation Mechanisms ofFrontiers in microbiology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Bacterial manganese(II) oxidation impacts the redox cycling of Mn, other elements, and compounds in the environment; therefore, it is important to understand the mechanisms of and enzymes responsible for Mn(II) oxidation. In several Mn(II)-oxidizing organisms, the identified Mn(II) oxidase belongs to either the multicopper oxidase (MCO) or the heme peroxidase family of proteins. However, the identity of the oxidase in Pseudomonas putida GB-1 has long remained unknown. To identify the P. putida GB-1 oxidase, we searched its genome and found several homologues of known or suspected Mn(II) oxidase-encoding genes (mnxG, mofA, moxA, and mopA). To narrow this list, we assumed that the Mn(II) oxidase gene would be conserved among Mn(II)-oxidizing pseudomonads but not in nonoxidizers and performed a genome comparison to 11 Pseudomonas species. We further assumed that the oxidase gene would be regulated by MnxR, a transcription factor required for Mn(II) oxidation. Two loci met all these criteria: PputGB1_2447, which encodes an MCO homologous to MnxG, and PputGB1_2665, which encodes an MCO with very low homology to MofA. In-frame deletions of each locus resulted in strains that retained some ability to oxidize Mn(II) or Mn(III); loss of oxidation was attained only upon deletion of both genes. These results suggest that PputGB1_2447 and PputGB1_2665 encode two MCOs that are independently capable of oxidizing both Mn(II) and Mn(III). The purpose of this redundancy is unclear; however, differences in oxidation phenotype for the single mutants suggest specialization in function for the two enzymes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.