ArticleThe Journal of biological chemistry2012
Localization and substrate selectivity of sea urchin multidrug (MDR) efflux transporters.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 73 citations in OpenAlex.
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- An IMPDH2 variant associated with neurodevelopmental disorder disrupts purine biosynthesis and somite organization.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Characterization of cellular and molecular immune components of the painted white sea urchin Lytechinus pictus in response to bacterial infection.Immunology and cell biology · 2025Article
- Genetic requirement ofeLife · 2024Article
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- The roles of ABCB1/P-glycoprotein drug transporters in regulating gut microbes and inflammation: insights from animal models, old and new.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Semi-automated, high-content imaging of drug transporter knockout sea urchin (Lytechinus pictus) embryos.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2024Article
- MCPIP1 functions as a safeguard of early embryonic development.Scientific reports · 2023Article
- The evolutionary history of Brachyury genes in Hydrozoa involves duplications, divergence, and neofunctionalization.Scientific reports · 2023Article
- Molecular mechanisms of tubulogenesis revealed in the sea star hydro-vascular organ.Nature communications · 2023Article
- Controlled uptake of PFOA in adult specimens of Paracentrotus lividus and evaluation of gene expression in their gonads and embryos.Environmental science and pollution research international · 2023Article
- Generation of a homozygous mutant drug transporter (ABCB1) knockout line in the sea urchin Lytechinus pictus.Development (Cambridge, England) · 2022Article
- A conserved node in the regulation of Vasa between an induced and an inherited program of primordial germ cell specification.Developmental biology · 2022Article
- Live imaging of echinoderm embryos to illuminate evo-devo.Frontiers in cell and developmental biology · 2022Article
- Polarized Dishevelled dissolution and reassembly drives embryonic axis specification in sea star oocytes.Current biology : CB · 2021Article
- Selective dephosphorylation by PP2A-B55 directs the meiosis I-meiosis II transition in oocytes.eLife · 2021Article
- Early patterning of ABCB, ABCC, and ABCG transporters establishes unique territories of small molecule transport in embryonic mesoderm and endoderm.Developmental biology · 2021Article
- CRISPR/Cas9 mutagenesis reveals a role for ABCB1 in gut immune responses to Vibrio diazotrophicus in sea urchin larvae.The Journal of experimental biology · 2021Article
- Citrullination regulates wound responses and tissue regeneration in zebrafish.The Journal of cell biology · 2020Article
- Quiescent Cells Actively Replenish CENP-A Nucleosomes to Maintain Centromere Identity and Proliferative Potential.Developmental cell · 2019Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
In this study, we cloned, expressed and functionally characterized Stronglycentrotus purpuratus (Sp) ATP-binding cassette (ABC) transporters. This screen identified three multidrug resistance (MDR) transporters with functional homology to the major types of MDR transporters found in humans. When overexpressed in embryos, the apical transporters Sp-ABCB1a, ABCB4a, and ABCG2a can account for as much as 87% of the observed efflux activity, providing a robust assay for their substrate selectivity. Using this assay, we found that sea urchin MDR transporters export canonical MDR susbtrates such as calcein-AM, bodipy-verapamil, bodipy-vinblastine, and mitoxantrone. In addition, we characterized the impact of nonconservative substitutions in the primary sequences of drug binding domains of sea urchin versus murine ABCB1 by mutation of Sp-ABCB1a and treatment of embryos with stereoisomeric cyclic peptide inhibitors (QZ59 compounds). The results indicated that two substitutions in transmembrane helix 6 reverse stereoselectivity of Sp-ABCB1a for QZ59 enantiomers compared with mouse ABCB1a. This suggests that subtle changes in the primary sequence of transporter drug binding domains could fine-tune substrate specificity through evolution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.