ArticleJournal of bacteriology2013
The Bacillus subtilis response regulator gene degU is positively regulated by CcpA and by catabolite-repressed synthesis of ClpC.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Microbial Proteases: Integration into Food Products and Industrial Processes.Current microbiology · 2025Review
- RNA-seq analysis identified glucose-responsive genes and YqfO as a global regulator in Bacillus subtilis.BMC research notes · 2021Article
- Surfactin, a quorum sensing signal molecule, globally affects the carbon metabolism inMetabolic engineering communications · 2021Article
- Rampant loss of social traits during domestication of a Bacillus subtilis natural isolate.Scientific reports · 2020Article
- The ComX Quorum Sensing Peptide ofMicroorganisms · 2020Article
- Analysis of a bac operon-silenced strain suggests pleiotropic effects of bacilysin in Bacillus subtilis.Journal of microbiology (Seoul, Korea) · 2020Article
- Surfactin: A Quorum-Sensing Signal Molecule to Relieve CCR inFrontiers in microbiology · 2020Article
- Glucose-Mediated Protein Arginine Phosphorylation/Dephosphorylation RegulatesFrontiers in microbiology · 2020Article
- Genetic variants of the oppA gene are involved in metabolic regulation of surfactin in Bacillus subtilis.Microbial cell factories · 2019Article
- Poly-γ-Glutamic Acids Contribute to Biofilm Formation and Plant Root Colonization in Selected Environmental Isolates ofFrontiers in microbiology · 2016Article
- YvoA and CcpA Repress the Expression of chiB in Bacillus thuringiensis.Applied and environmental microbiology · 2015Article
- Novel Routes for Improving Biocontrol Activity of Bacillus Based Bioinoculants.Frontiers in microbiology · 2015Review
- Bacterial competition reveals differential regulation of the pks genes by Bacillus subtilis.Journal of bacteriology · 2014Article
- Regulation of the response regulator gene degU through the binding of SinR/SlrR and exclusion of SinR/SlrR by DegU in Bacillus subtilis.Journal of bacteriology · 2014Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Bacillus subtilis, the response regulator DegU and its cognate kinase, DegS, constitute a two-component system that regulates many cellular processes, including exoprotease production and genetic competence. Phosphorylated DegU (DegU-P) activates its own promoter and is degraded by the ClpCP protease. We observed induction of degU by glucose in sporulation medium. This was abolished in two mutants: the ccpA (catabolite control protein A) and clpC disruptants. Transcription of the promoter of the operon containing clpC (PclpC) decreased in the presence of glucose, and the disruption of ccpA resulted in derepression of PclpC. However, this was not directly mediated by CcpA, because we failed to detect binding of CcpA to PclpC. Glucose decreased the expression of clpC, leading to low cellular concentrations of the ClpCP protease. Thus, degU is induced through activation of autoregulation by a decrease in ClpCP-dependent proteolysis of DegU-P. An electrophoretic mobility shift assay showed that CcpA bound directly to the degU upstream region, indicating that CcpA activates degU through binding. The bound region was narrowed down to 27 bases, which contained a cre (catabolite-responsive element) sequence with a low match to the cre consensus sequence. In a footprint analysis, CcpA specifically protected a region containing the cre sequence from DNase I digestion. The induction of degU by glucose showed complex regulation of the degU gene.
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