ArticleNature2012
Structure of the Mediator head module.
Article in Nature, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 99 citations in OpenAlex.
- PsAvh109 suppresses SA-triggered immunity by mimicking TPL function to disrupt mediator complex assembly.Nature communications · 2026Article
- Transcription-replication conflict resolution by nuclear RNA interference.Molecular cell · 2025Article
- A quantitative comparison of the deleteriousness of missense and nonsense mutations using the structurally resolved human protein interactome.Protein science : a publication of the Protein Society · 2025Article
- Identification of the role of MED6 in the development and prognosis of lung adenocarcinoma based on multi-omics profiling.Journal of Cancer · 2025Article
- Yeast PIC-Mediator structure with RNA polymerase II C-terminal domain.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The Mediator complex as a master regulator of transcription by RNA polymerase II.Nature reviews. Molecular cell biology · 2022Review
- Assembly of RNA polymerase II transcription initiation complexes.Current opinion in structural biology · 2022Review
- Comparison of transcriptional initiation by RNA polymerase II across eukaryotic species.eLife · 2021Article
- Profile of Patrick Cramer.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Article
- MedProDB: A database of Mediator proteins.Computational and structural biotechnology journal · 2021Article
- Structure and mechanism of the RNA polymerase II transcription machinery.Genes & development · 2020Review
- Functional interplay between Mediator and RNA polymerase II in Rad2/XPG loading to the chromatin.Nucleic acids research · 2019Article
- Interaction map of Arabidopsis Mediator complex expounding its topology.Nucleic acids research · 2019Article
- Role of integrative structural biology in understanding transcriptional initiation.Methods (San Diego, Calif.) · 2019Review
- Twenty years of Mediator complex structural studies.Biochemical Society transactions · 2019Review
- The Structures of Eukaryotic Transcription Pre-initiation Complexes and Their Functional Implications.Sub-cellular biochemistry · 2019Review
- The complex structure and function of Mediator.The Journal of biological chemistry · 2018Review
- Crystal structure of human Mediator subunit MED23.Nature communications · 2018Article
- Transcription regulation by the Mediator complex.Nature reviews. Molecular cell biology · 2018Review
3 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene transcription by RNA polymerase (Pol) II requires the coactivator complex Mediator. Mediator connects transcriptional regulators and Pol II, and is linked to human disease. Mediator from the yeast Saccharomyces cerevisiae has a molecular mass of 1.4 megadaltons and comprises 25 subunits that form the head, middle, tail and kinase modules. The head module constitutes one-half of the essential Mediator core, and comprises the conserved subunits Med6, Med8, Med11, Med17, Med18, Med20 and Med22. Recent X-ray analysis of the S. cerevisiae head module at 4.3 Å resolution led to a partial architectural model with three submodules called neck, fixed jaw and moveable jaw. Here we determine de novo the crystal structure of the head module from the fission yeast Schizosaccharomyces pombe at 3.4 Å resolution. Structure solution was enabled by new structures of Med6 and the fixed jaw, and previous structures of the moveable jaw and part of the neck, and required deletion of Med20. The S. pombe head module resembles the head of a crocodile with eight distinct elements, of which at least four are mobile. The fixed jaw comprises tooth and nose domains, whereas the neck submodule contains a helical spine and one limb, with shoulder, arm and finger elements. The arm and the essential shoulder contact other parts of Mediator. The jaws and a central joint are implicated in interactions with Pol II and its carboxy-terminal domain, and the joint is required for transcription in vitro. The S. pombe head module structure leads to a revised model of the S. cerevisiae module, reveals a high conservation and flexibility, explains known mutations, and provides the basis for unravelling a central mechanism of gene regulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.