ArticleNeural development2012
Zebrafish Mnx proteins specify one motoneuron subtype and suppress acquisition of interneuron characteristics.
Article in Neural development, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 63 citations in OpenAlex.
- The Prospective Regulatory Functions of lncRNAs and Their ceRNA Networks in the Development of Motor Neurons and Associated Diseases.Biomolecules · 2026Review
- Single-cell transcriptomic profiling of zebrafish primary motor neurons reveals subtype-specific gene expression signatures.iScience · 2025Article
- Novel Transgenic Zebrafish Lines to Study the CHRNA3-B4-A5 Gene Cluster.Developmental neurobiology · 2025Article
- Electrical Synapses Mediate Embryonic Hyperactivity in a Zebrafish Model of Fragile X Syndrome.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Lhx4 surpasses its paralog Lhx3 in promoting the differentiation of spinal V2a interneurons.Cellular and molecular life sciences : CMLS · 2024Article
- In Vivo Optogenetic Phase Transition of an Intrinsically Disordered Protein.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Evaluating the association of biallelic OGDHL variants with significant phenotypic heterogeneity.Genome medicine · 2023Article
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- Single-cell transcriptomic analysis reveals diversity within mammalian spinal motor neurons.Nature communications · 2023Article
- Temperature- and chemical-induced neurotoxicity in zebrafish.Frontiers in physiology · 2023Review
- NemoTrainer: Automated Conditioning for Stimulus-Directed Navigation and Decision Making in Free-Swimming Zebrafish.Animals : an open access journal from MDPI · 2022Article
- A deep-dive into fictive locomotion - a strategy to probe cellular activity during speed transitions in fictively swimming zebrafish larvae.Biology open · 2022Article
- Establishing the Molecular and Functional Diversity of Spinal Motoneurons.Advances in neurobiology · 2022Article
- Fmrp regulates neuronal balance in embryonic motor circuit formation.Frontiers in neuroscience · 2022Article
- Unique advantages of zebrafish larvae as a model for spinal cord regeneration.Frontiers in molecular neuroscience · 2022Review
- Automated in vivo drug screen in zebrafish identifies synapse-stabilising drugs with relevance to spinal muscular atrophy.Disease models & mechanisms · 2021Article
- Orderly compartmental mapping of premotor inhibition in the developing zebrafish spinal cord.Science (New York, N.Y.) · 2020Article
- Dynamic regulation of the cholinergic system in the spinal central nervous system.Scientific reports · 2020Article
- Epigenetic factors Dnmt1 and Uhrf1 coordinate intestinal development.Developmental biology · 2019Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundPrecise matching between motoneuron subtypes and the muscles they innervate is a prerequisite for normal behavior. Motoneuron subtype identity is specified by the combination of transcription factors expressed by the cell during its differentiation. Here we investigate the roles of Mnx family transcription factors in specifying the subtypes of individually identified zebrafish primary motoneurons.
resultsZebrafish has three Mnx family members. We show that each of them has a distinct and temporally dynamic expression pattern in each primary motoneuron subtype. We also show that two Mnx family members are expressed in identified VeLD interneurons derived from the same progenitor domain that generates primary motoneurons. Surprisingly, we found that Mnx proteins appear unnecessary for differentiation of VeLD interneurons or the CaP motoneuron subtype. Mnx proteins are, however, required for differentiation of the MiP motoneuron subtype. We previously showed that MiPs require two temporally-distinct phases of Islet1 expression for normal development. Here we show that in the absence of Mnx proteins, the later phase of Islet1 expression is initiated but not sustained, and MiPs become hybrids that co-express morphological and molecular features of motoneurons and V2a interneurons. Unexpectedly, these hybrid MiPs often extend CaP-like axons, and some MiPs appear to be entirely transformed to a CaP morphology.
conclusionsOur results suggest that Mnx proteins promote MiP subtype identity by suppressing both interneuron development and CaP axon pathfinding. This is, to our knowledge, the first report of transcription factors that act to distinguish CaP and MiP subtype identities. Our results also suggest that MiP motoneurons are more similar to V2 interneurons than are CaP motoneurons.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.