ArticleBMC complementary and alternative medicine2012
Novel molecular, cytotoxical, and immunological study on promising and selective anticancer activity of mung bean sprouts.
Article in BMC complementary and alternative medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 43 citations in OpenAlex.
- The Effect of Illumination Patterns during Mung Bean Seed Germination on the Metabolite Composition of the Sprouts.Plants (Basel, Switzerland) · 2023Article
- Current therapeutic modalities and chemopreventive role of natural products in liver cancer: Progress and promise.World journal of hepatology · 2023Review
- The Effect ofPlants (Basel, Switzerland) · 2021Article
- A review on metabolites and pharmaceutical potential of food legume crop mung bean (Biotechnologia · 2021Review
- Germination Improves the Polyphenolic Profile and Functional Value of Mung Bean (Antioxidants (Basel, Switzerland) · 2020Article
- Mung Bean (Nutrients · 2019Review
- Inhibitory Effects of Pulse Bioactive Compounds on Cancer Development Pathways.Diseases (Basel, Switzerland) · 2018Review
- Article
- RANDOMIZED ANTICANCER AND CYTOTOXICITY ACTIVITIES OFAfrican journal of traditional, complementary, and alternative medicines : AJTCAM · 2017Article
- Selective cytotoxicity of a Vietnamese traditional formula, Nam Dia long, against MCF-7 cells by synergistic effects.BMC complementary and alternative medicine · 2016Article
- Review
- Apoptosis Induction by Polygonum minus is related to antioxidant capacity, alterations in expression of apoptotic-related genes, and S-phase cell cycle arrest in HepG2 cell line.BioMed research international · 2014Article
- In Vivo Immunomodulation and Lipid Peroxidation Activities Contributed to Chemoprevention Effects of Fermented Mung Bean against Breast Cancer.Evidence-based complementary and alternative medicine : eCAM · 2013Article
- Natural Products Mediated Targeting of Virally Infected Cancer.Dose-response : a publication of International Hormesis SocietyReview
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe anticancer and immunomodulatory activity of mung bean sprouts (MBS) and the underlying mechanisms against human cervical and hepatocarcinoma cancer cells were explored.
methodsMBS cytotoxicity and MBS-induced anticancer cytokines, TNF-α and IFN-β from cancer cells, and immunological cytokines, IL-4, IFN-γ, and IL-10 from peripheral mononuclear cells (PMNC) were assessed by MTS and ELISA assays. Apoptotic cells were investigated by flow cytometry. The expression level of apoptotic genes (Bax, BCL-2, Capsases 7-9) and cell cycle regulatory genes (cyclin D, E, and A) and tumor suppressor proteins (p27, p21, and p53) was assessed by real-time qPCR in the cancer cells treated with extract IC50.
resultsThe cytotoxicity on normal human cells was significantly different from HeLa and HepG2 cells, 163.97 ± 5.73, 13.3 ± 0.89, and 14.04 ± 1.5 mg/ml, respectively. The selectivity index (SI) was 12.44 ± 0.83 for HeLa and 11.94 ± 1.2 for HepG2 cells. Increased levels of TNF-α and IFN-β were observed in the treated HeLa and HepG2 culture supernatants when compared with untreated cells. MBS extract was shown to be an immunopolarizing agent by inducing IFNγ and inhibiting IL-4 production by PBMC; this leads to triggering of CMI and cellular cytotoxicity. The extract induced apoptosis, in a dose and time dependent manner, in treated HeLa and HepG2, but not in untreated, cells (P < 0.05). The treatment significantly induced cell cycle arrest in G0/G1 in HeLa cells. The percentage of cells in G0/G1 phase of the treated HeLa cells increased from 62.87 ± 2.1%, in untreated cells, to 80.48 ± 2.97%. Interestingly, MBS IC50 induced the expression of apoptosis and tumor suppressor related genes in both HeLa and HepG2 cells. MBS extract succeeded in inducing cdk-inhibitors, p21, p53, and p27 in HeLa cells while it induced only p53 in HepG2 cells (P < 0.05). This is a clue for the cell type- specific interaction of the studied extract. These proteins inhibit the cyclin-cdk complexes apart from the presence of some other components that might stimulate some cyclins such as cyclin E, A, and D.
conclusionMBS extract was shown to be a potent anticancer agent granting new prospects of anticancer therapy using natural products.
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