ArticleAnalytical chemistry2012
Highly efficient binding of paramagnetic beads bioconjugated with 100,000 or more antibodies to protein-coated surfaces.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 50 citations in OpenAlex.
- Detrimental effect of metal contaminants on forensic DNA analysis and strategies for mitigation.International journal of legal medicine · 2026Review
- Bio-Inspired Strategies for Improving the Selectivity and Sensitivity of Artificial Noses: A Review.Sensors (Basel, Switzerland) · 2020Review
- Indirect determination of mercury(II) by using magnetic nanoparticles, CdS quantum dots and mercury(II)-binding aptamers, and quantitation of released CdS by graphite furnace AAS.Mikrochimica acta · 2020Article
- Restricted Proteolysis and LC-MS/MS To Evaluate the Orientation of Surface-Immobilized Antibodies.Analytical chemistry · 2019Article
- Epitope-Resolved Detection of Peanut-Specific IgE Antibodies by Surface Plasmon Resonance Imaging.Chembiochem : a European journal of chemical biology · 2018Article
- Albumin removal from human serum using surface nanopockets on silica-coated magnetic nanoparticles.Chemical communications (Cambridge, England) · 2017Article
- FeBiosensors & bioelectronics · 2017Article
- Microchip-based ultrafast serodiagnostic assay for tuberculosis.Scientific reports · 2016Article
- High-Throughput Electrochemical Microfluidic Immunoarray for Multiplexed Detection of Cancer Biomarker Proteins.ACS sensors · 2016Article
- Click Chemistry-Mediated Nanosensors for Biochemical Assays.Theranostics · 2016Review
- Antibody-like Biorecognition Sites for Proteins from Surface Imprinting on Nanoparticles.ACS applied materials & interfaces · 2015Article
- Low-cost photolithographic fabrication of nanowires and microfilters for advanced bioassay devices.Sensors (Basel, Switzerland) · 2015Article
- Ultrasensitive carbohydrate-peptide SPR imaging microarray for diagnosing IgE mediated peanut allergy.The Analyst · 2014Article
- Glucose oxidase-catalyzed growth of gold nanoparticles enables quantitative detection of attomolar cancer biomarkers.Analytical chemistry · 2014Article
- On-line protein capture on magnetic beads for ultrasensitive microfluidic immunoassays of cancer biomarkers.Biosensors & bioelectronics · 2014Article
- Nanomaterials and biomaterials in electrochemical arrays for protein detection.Journal of materials chemistry. B · 2014Article
- Acetylcholinesterase-catalyzed hydrolysis allows ultrasensitive detection of pathogens with the naked eye.Angewandte Chemie (International ed. in English) · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
We report here the first kinetic characterization of 1 μm diameter superparamagnetic particles (MP) decorated with over 100,000 antibodies binding to protein antigens attached to flat surfaces. Surface plasmon resonance (SPR) was used to show that these antibody-derivatized MPs (MP-Ab(2)) exhibit irreversible binding with 100-fold increased association rates compared to free antibodies. The estimated upper limit for the dissociation constant of MP-Ab(2) from the SPR sensor surface is 5 fM, compared to 3-8 nM for the free antibodies. These results are explained by up to 2000 interactions of MP-Ab(2) with protein-decorated surfaces. Findings are consistent with highly efficient capture of protein antigens in solution by the MP-Ab(2) and explain in part the utility of these beads for ultrasensitive protein detection into the fM and aM range. Aggregation of these particles on the SPR chip, probably due to residual magnetic microdomains in the particles, also contributes to ultrasensitive detection and may also help drive the irreversible binding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.