ArticleBiochemistry research international2012
A critical appraisal of quantitative studies of protein degradation in the framework of cellular proteostasis.
Article in Biochemistry research international, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Protein degradation kinetics measured by microinjection and live-cell fluorescence microscopy.Scientific reports · 2024Article
- Mitochondrial LonP1 protease is implicated in the degradation of unstable Parkinson's disease-associated DJ-1/PARK 7 missense mutants.Scientific reports · 2021Article
- Image-Based Analysis of Protein Stability.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2020Article
- Physiological and pathophysiological characteristics of ataxin-3 isoforms.The Journal of biological chemistry · 2019Article
- Lysine-Less Variants of Spinal Muscular Atrophy SMN and SMNΔ7 Proteins Are Degraded by the Proteasome Pathway.International journal of molecular sciences · 2017Article
- Incompatibility of chemical protein synthesis inhibitors with accurate measurement of extended protein degradation rates.Pharmacology research & perspectives · 2017Article
- Neuroprotective Strategy in Retinal Degeneration: Suppressing ER Stress-Induced Cell Death via Inhibition of the mTOR Signal.International journal of molecular sciences · 2017Review
- Quantitative chemical proteomics profiling of de novo protein synthesis during starvation-mediated autophagy.Autophagy · 2016Article
- Cycloheximide Treatment Causes a ZVAD-Sensitive Protease-Dependent Cleavage of Human Tau in Drosophila Cells.Journal of Alzheimer's disease : JAD · 2016Article
- Putative Breast Cancer Driver Mutations in TBX3 Cause Impaired Transcriptional Repression.Frontiers in oncology · 2015Article
- Mutations in MAB21L2 result in ocular Coloboma, microcornea and cataracts.PLoS genetics · 2015Article
- Article
- Proteome dynamics: revisiting turnover with a global perspective.Molecular & cellular proteomics : MCP · 2012Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein homeostasis, proteostasis, is essential to understand cell function. Protein degradation is a crucial component of the proteostatic mechanisms of the cell. Experiments on protein degradation are nowadays present in many investigations in the field of molecular and cell biology. In the present paper, we focus on the different experimental approaches to study protein degradation and present a critical appraisal of the results derived from steady-state and kinetic experiments using detection of unlabelled and labelled protein methodologies with a proteostatic perspective. This perspective allows pinpointing the limitations in interpretation of results and the need of further experiments and/or controls to establish "definitive evidence" for the role of protein degradation in the proteostasis of a given protein or the entire proteome. We also provide a spreadsheet for simple calculations of mRNA and protein decays for mimicking different experimental conditions and a checklist for the analysis of experiments dealing with protein degradation studies that may be useful for researchers interested in the area of protein turnover.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.