ArticlePloS one2012
C. difficile 630Δerm Spo0A regulates sporulation, but does not contribute to toxin production, by direct high-affinity binding to target DNA.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- A single DNA methylation site regulates cell fate during Clostridioides difficile sporulation.PLoS pathogens · 2026Article
- Cleavage of an engulfment peptidoglycan hydrolase by a sporulation signature protease in Clostridioides difficile.Molecular microbiology · 2024Article
- Spores of Clostridioides difficile are toxin delivery vehicles.Communications biology · 2024Article
- Review
- Clostridioides difficile Sporulation.Advances in experimental medicine and biology · 2024Review
- Identification of DraRS inJournal of bacteriology · 2023Article
- The RgaS-RgaR two-component system promotes Clostridioides difficile sporulation through a small RNA and the Agr1 system.PLoS genetics · 2023Article
- The RgaS-RgaR two-component system promotesbioRxiv : the preprint server for biology · 2023Article
- A network of small RNAs regulates sporulation initiation in Clostridioides difficile.The EMBO journal · 2023Article
- Regulatory transcription factors ofCritical reviews in microbiology · 2023Review
- Article
- Review
- Response Regulator CD1688 Is a Negative Modulator of Sporulation in Clostridioides difficile.Journal of bacteriology · 2022Article
- Identification of Functional Spo0A Residues Critical for Sporulation in Clostridioides difficile.Journal of molecular biology · 2022Article
- Three Orphan Histidine Kinases Inhibit Clostridioides difficile Sporulation.Journal of bacteriology · 2022Article
- Genetic mechanisms governing sporulation initiation in Clostridioides difficile.Current opinion in microbiology · 2022Review
- Regulation of Clostridioides difficile toxin production.Current opinion in microbiology · 2022Review
- Practical observations on the use of fluorescent reporter systems in Clostridioides difficile.Antonie van Leeuwenhoek · 2022Article
- Article
- CD25890, a conserved protein that modulates sporulation initiation in Clostridioides difficile.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clostridium difficile is a Gram positive, anaerobic bacterium that can form highly resistant endospores. The bacterium is the causative agent of C. difficile infection (CDI), for which the symptoms can range from a mild diarrhea to potentially fatal pseudomembranous colitis and toxic megacolon. Endospore formation in Firmicutes, including C. difficile, is governed by the key regulator for sporulation, Spo0A. In Bacillus subtilis, this transcription factor is also directly or indirectly involved in various other cellular processes. Here, we report that C. difficile Spo0A shows a high degree of similarity to the well characterized B. subtilis protein and recognizes a similar binding sequence. We find that the laboratory strain C. difficile 630Δerm contains an 18bp-duplication near the DNA-binding domain compared to its ancestral strain 630. In vitro binding assays using purified C-terminal DNA binding domain of the C. difficile Spo0A protein demonstrate direct binding to DNA upstream of spo0A and sigH, early sporulation genes and several other putative targets. In vitro binding assays suggest that the gene encoding the major clostridial toxin TcdB may be a direct target of Spo0A, but supernatant derived from a spo0A negative strain was no less toxic towards Vero cells than that obtained from a wild type strain, in contrast to previous reports. These results identify for the first time direct (putative) targets of the Spo0A protein in C. difficile and make a positive effect of Spo0A on production of the large clostridial toxins unlikely.
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