ArticleMarine drugs2012
Isolation and characterization of a lycopene ε-cyclase gene of Chlorella (Chromochloris) zofingiensis. Regulation of the carotenogenic pathway by nitrogen and light.
Article in Marine drugs, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 63 citations in OpenAlex.
- Astaxanthin fromMarine drugs · 2025Review
- Characterization of microalgal β-carotene and astaxanthin: exploring their health-promoting properties under the effect of salinity and light intensity.Biotechnology for biofuels and bioproducts · 2025Review
- Distribution, Biosynthesis, and Function of Carotenoids in Oxygenic Phototrophic Algae.Marine drugs · 2025Review
- Microbial chassis as the platform for production of dihydroxy xanthophyll-based carotenoids: an overview of recent advances in biomanufacturing.World journal of microbiology & biotechnology · 2024Review
- Article
- Progress towards a targeted biorefinery ofBiomass conversion and biorefinery · 2024Review
- Functional Characterization of Lycopene β- and ε-Cyclases from a Lutein-Enriched Green MicroalgaMarine drugs · 2023Article
- Microalgal Carotenoids: Therapeutic Application and Latest Approaches to Enhance the Production.Current issues in molecular biology · 2022Review
- Genome-based identification and comparative analysis of enzymes for carotenoid biosynthesis in microalgae.World journal of microbiology & biotechnology · 2021Article
- Disparity of the carotenoids antioxidant properties of wild-type andPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Diverse Biosynthetic Pathways and Protective Functions against Environmental Stress of Antioxidants in Microalgae.Plants (Basel, Switzerland) · 2021Review
- The oleaginous astaxanthin-producing alga Chromochloris zofingiensis: potential from production to an emerging model for studying lipid metabolism and carotenogenesis.Biotechnology for biofuels · 2021Review
- Astaxanthin Is Ketolated from Zeaxanthin Independent of Fatty Acid Synthesis inPlant physiology · 2020Article
- Algae-Derived Bioactive Compounds with Anti-Lung Cancer Potential.Marine drugs · 2020Review
- Microalgal Enzymes with Biotechnological Applications.Marine drugs · 2019Review
- Time-resolved carotenoid profiling and transcriptomic analysis reveal mechanism of carotenogenesis for astaxanthin synthesis in the oleaginous green algaBiotechnology for biofuels · 2019Article
- Comparative Analysis of Culture Conditions for the Optimization of Carotenoid Production in Several Strains of the Picoeukaryote Ostreococcus.Marine drugs · 2018Article
- High-value biomass from microalgae production platforms: strategies and progress based on carbon metabolism and energy conversion.Biotechnology for biofuels · 2018Review
- Rapid Estimation of Astaxanthin and the Carotenoid-to-Chlorophyll Ratio in the Green Microalga Chromochloris zofingiensis Using Flow Cytometry.Marine drugs · 2017Article
- Functional Characterization of a Missing Branch Component inFrontiers in plant science · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The isolation and characterization of the lycopene ε-cyclase gene from the green microalga Chlorella (Chromochloris) zofingiensis (Czlcy-e) was performed. This gene is involved in the formation of the carotenoids α-carotene and lutein. Czlcy-e gene encoded a polypeptide of 654 amino acids. A single copy of Czlcy-e was found in C. zofingiensis. Functional analysis by heterologous complementation in Escherichia coli showed the ability of this protein to convert lycopene to δ-carotene. In addition, the regulation of the carotenogenic pathway by light and nitrogen was also studied in C. zofingiensis. High irradiance stress did not increase mRNA levels of neither lycopene β-cyclase gene (lcy-b) nor lycopene ε-cyclase gene (lcy-e) as compared with low irradiance conditions, whereas the transcript levels of psy, pds, chyB and bkt genes were enhanced, nevertheless triggering the synthesis of the secondary carotenoids astaxanthin, canthaxanthin and zeaxanthin and decreasing the levels of the primary carotenoids α-carotene, lutein, violaxanthin and β-carotene. Nitrogen starvation per se enhanced mRNA levels of all genes considered, except lcy-e and pds, but did not trigger the synthesis of astaxanthin, canthaxanthin nor zeaxanthin. The combined effect of both high light and nitrogen starvation stresses enhanced significantly the accumulation of these carotenoids as well as the transcript levels of bkt gene, as compared with the effect of only high irradiance stress.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.