ArticleCardiovascular research2013
Myosin filament assembly onto myofibrils in live neonatal cardiomyocytes observed by TPEF-SHG microscopy.
Article in Cardiovascular research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Quantifying Regional and Age-Dependent Microstructural Changes in Porcine Ventricles from Neonatal to Adulthood Using DT-MRI and TPEF-SHG Microscopy.Annals of biomedical engineering · 2026Article
- From function to structure: how myofibrillogenesis influences the transverse-axial tubular system development and its peculiarities.Frontiers in physiology · 2025Review
- Sarcomere maturation: function acquisition, molecular mechanism, and interplay with other organelles.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Constitutive activation of ERK1/2 signaling protects against myocardial ischemia via inhibition of mitochondrial fragmentation in the aging heart.Annals of translational medicine · 2021Article
- Diet alters age-related remodeling of aortic collagen in mice susceptible to atherosclerosis.American journal of physiology. Heart and circulatory physiology · 2021Article
- Extracellular Matrix in Regulation of Contractile System in Cardiomyocytes.International journal of molecular sciences · 2019Review
- Region-Specific Microstructure in the Neonatal Ventricles of a Porcine Model.Annals of biomedical engineering · 2018Article
- Cardiotrophic Growth Factor-Driven Induction of Human Muse Cells Into Cardiomyocyte-Like Phenotype.Cell transplantation · 2018Article
- The cardiac-restricted protein ADP-ribosylhydrolase-like 1 is essential for heart chamber outgrowth and acts on muscle actin filament assembly.Developmental biology · 2016Article
- Disassembly of myofibrils and potential imbalanced forces on Z-discs in cultured adult cardiomyocytes.Cytoskeleton (Hoboken, N.J.) · 2016Article
- Dynamic Myofibrillar Remodeling in Live Cardiomyocytes under Static Stretch.Scientific reports · 2016Article
- Comparison of Aortic Collagen Fiber Angle Distribution in Mouse Models of Atherosclerosis Using Second-Harmonic Generation (SHG) Microscopy.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2016Article
- Multimodal SHG-2PF Imaging of Microdomain Ca2+-Contraction Coupling in Live Cardiac Myocytes.Circulation research · 2016Review
- Interactive relationship between basement-membrane development and sarcomerogenesis in single cardiomyocytes.Experimental cell research · 2015Article
- Jasplakinolide reduces actin and tropomyosin dynamics during myofibrillogenesis.Cytoskeleton (Hoboken, N.J.) · 2014Article
- Localization of sarcomeric proteins during myofibril assembly in cultured mouse primary skeletal myotubes.Anatomical record (Hoboken, N.J. : 2007) · 2014Article
- Does Nebulin Make Tropomyosin Less Dynamic in Mature Myofibrils in Cross-Striated Myocytes?Journal of cytology & histology · 2014Article
- Role of the basement membrane in regulation of cardiac electrical properties.Annals of biomedical engineering · 2014Article
- Pulse splitter-based nonlinear microscopy for live-cardiomyocyte imaging.Proceedings of SPIE--the International Society for Optical Engineering · 2014Article
- A novel approach for assessing cardiac fibrosis using label-free second harmonic generation.The international journal of cardiovascular imaging · 2013Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
Abstract
aimsUnderstanding myofibrillogenesis is essential for elucidating heart muscle formation, development, and remodelling in response to physiological stimulation. Here, we report the dynamic assembly process of contractile myosin filaments onto myofibrils in a live cardiomyocyte culture during myofibrillogenesis. METHODS AND
resultsUtilizing a custom-built, two-photon excitation fluorescence and second harmonic generation imaging system equipped with an on-stage incubator, we observed new sarcomere additions in rat neonatal cardiomyocytes during 10 h of on-stage incubation. The new sarcomere additions occurred at the side of existing myofibrils, where we observed mature myofibrils acting as templates, or at the interstice of several separated myofibrils.
conclusionsDuring sarcomeric addition, myosin filaments are assembled onto the premyofibril laterally. This lateral addition, which proceeds stepwise along the axial direction, plays an important role in the accumulation of Z-bodies to form mature Z-disks and in the regulation of sarcomeric alignment during maturation.
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Registered trials
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