ArticleJournal of visualized experiments : JoVE2012
Patch clamp recordings in inner ear hair cells isolated from zebrafish.
Article in Journal of visualized experiments : JoVE, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Article
- In Vivo Calcium Imaging of Lateral-line Hair Cells in Larval Zebrafish.Journal of visualized experiments : JoVE · 2018Article
- Patch-clamp recordings from lateral line neuromast hair cells of the living zebrafish.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- The role of slingshot-1L (SSH1L) in the differentiation of human bone marrow mesenchymal stem cells into cardiomyocyte-like cells.Molecules (Basel, Switzerland) · 2012Article
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Authors and funding
8 authors.
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Abstract
Patch clamp analyses of the voltage-gated channels in sensory hair cells isolated from a variety of species have been described previously(1-4) but this video represents the first application of those techniques to hair cells from zebrafish. Here we demonstrate a method to isolate healthy, intact hair cells from all of the inner ear end-organs: saccule, lagena, utricle and semicircular canals. Further, we demonstrate the diversity in hair cell size and morphology and give an example of the kinds of patch clamp recordings that can be obtained. The advantage of the use of this zebrafish model system over others stems from the availability of zebrafish mutants that affect both hearing and balance. In combination with the use of transgenic lines and other techniques that utilize genetic analysis and manipulation, the cell isolation and electrophysiological methods introduced here should facilitate greater insight into the roles hair cells play in mediating these sensory modalities.
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