ArticleBMJ open2012
Gene expression profiles of alveolar type II cells of chronic obstructive pulmonary disease: a case-control study.
Article in BMJ open, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
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- Integrative genomics analysis of various omics data and networks identify risk genes and variants vulnerable to childhood-onset asthma.BMC medical genomics · 2020Article
- PulmonDB: a curated lung disease gene expression database.Scientific reports · 2020Article
- Immunoproteasomes as a novel antiviral mechanism in rhinovirus-infected airways.Clinical science (London, England : 1979) · 2018Article
- A candidate gene identification strategy utilizing mouse to human big-data mining: "3R-tenet" in COPD genetic research.Respiratory research · 2018Article
- Long-term Air Pollution Exposure, Genome-wide DNA Methylation and Lung Function in the LifeLines Cohort Study.Environmental health perspectives · 2018Article
- Regulation of immunoproteasome function in the lung.Scientific reports · 2015Article
- Comprehensive analysis of gene-expression profile in chronic obstructive pulmonary disease.International journal of chronic obstructive pulmonary disease · 2015Article
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Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesThe aim of this study was to identify the gene expression pattern specific in alveolar epithelial type II cells (ATII cells) isolated from patients with chronic obstructive pulmonary disease (COPD).
designCase control.
settingTwo hospitals in Japan.
participantsThree patients without COPD and three patients with COPD in microarray analyses. Five smokers without COPD and nine smokers with COPD in the following analyses. PRIMARY AND SECONDARY OUTCOME MEASURED: Primary outcome included identification of differentially expressed genes and activated or inhibited pathways in ATII cells of the patients with COPD, compared to those of the patients without COPD, using Affymetrix gene expression arrays. Secondary outcome included validation of the results of microarray analyses by quantitative reverse transcription-PCR.
resultsWe isolated ATII cells from COPD and non-COPD lungs using fluorescence-activated cell sorting. We performed Affymetrix gene expression arrays on both types of ATII cells. Gene set enrichment analyses revealed that two major gene sets were enriched in ATII cells from COPD lungs: interferon-responsive gene sets and gene sets associated with cell cycle progression. Gene ontology term enrichment analyses indicated that among the interferon-stimulated genes, ATII cells in COPD expressed genes such as PSMB8, PSMB9, TAP1 and TAP2 associated with the antigen processing and presentation pathway. We validated the results of the microarray analyses using quantitative reverse transcriptase-PCR. In addition, FACS analysis indicated that the percentage of ATII cells to CD45-negative lung cells isolated from COPD lungs were significantly increased more than that from non-COPD lungs.
conclusionsOur study demonstrated that interferon-stimulated genes involved in the antigen processing and presentation pathway and genes involved in cell cycle progression were enriched in ATII cells of the patients with COPD. These pathways might alter phenotypes of ATII cells in COPD lungs.
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Registered trials
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