ArticleExperimental cell research2013
Fibroblast cluster formation on 3D collagen matrices requires cell contraction dependent fibronectin matrix organization.
Article in Experimental cell research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- Advanced Functional Wound Dressings in Precision Surgery: Immunometabolic Reprogramming, Bioadaptive Biomaterials, and Intelligent Regenerative Interfaces.International journal of molecular sciences · 2026Review
- Push or pull: how cytoskeletal crosstalk facilitates nuclear movement through 3D environments.Physical biology · 2022Review
- A suspended layer additive manufacturing approach to the bioprinting of tri-layered skin equivalents.APL bioengineering · 2021Article
- Acoustic Fabrication of Collagen-Fibronectin Composite Gels Accelerates Microtissue Formation.Applied sciences (Basel, Switzerland) · 2020Article
- Regulation of opticin on bioactivity of retinal vascular endothelial cells cultured in collagen.International journal of ophthalmology · 2020Article
- A 3D bioinspired highly porous polymeric scaffolding system forRSC advances · 2018Article
- Fibroblast-fibronectin patterning and network formation in 3D fibrin matrices.Matrix biology : journal of the International Society for Matrix Biology · 2017Article
- Emergent cellular self-organization and mechanosensation initiate follicle pattern in the avian skin.Science (New York, N.Y.) · 2017Article
- Vascular disease-causing mutation, smooth muscle α-actin R258C, dominantly suppresses functions of α-actin in human patient fibroblasts.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- 3D culture models of tissues under tension.Journal of cell science · 2017Review
- Collaboration of fibronectin matrix with other extracellular signals in morphogenesis and differentiation.Current opinion in cell biology · 2016Review
- Cooperative effects of fibronectin matrix assembly and initial cell-substrate adhesion strength in cellular self-assembly.Acta biomaterialia · 2016Article
- PDGF‑stimulated dispersal of cell clusters and disruption of fibronectin matrix on three-dimensional collagen matrices requires matrix metalloproteinase-2.Molecular biology of the cell · 2015Article
- The Role of Thrombin and Cell Contractility in Regulating Clustering and Collective Migration of Corneal Fibroblasts in Different ECM Environments.Investigative ophthalmology & visual science · 2015Article
- Collagen matrix as a tool in studying fibroblastic cell behavior.Cell adhesion & migration · 2015Review
- The different roles of myosin IIA and myosin IIB in contraction of 3D collagen matrices by human fibroblasts.Experimental cell research · 2014Article
- Article
- Techniques for assessing 3-D cell-matrix mechanical interactions in vitro and in vivo.Experimental cell research · 2013Review
- Fibroblast morphogenesis on 3D collagen matrices: the balance between cell clustering and cell migration.Experimental cell research · 2013Review
- Regional fibronectin and collagen fibril co-assembly directs cell proliferation and microtissue morphology.PloS one · 2013Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Fibroblasts incubated on 3D collagen matrices in serum or lysophosphatidic acid (LPA)-containing medium self-organize into clusters through a mechanism that requires cell contraction. However, in platelet-derived growth factor (PDGF)-containing medium, cells migrate as individuals and do not form clusters even though they constantly encounter each other. Here, we present evidence that a required function of cell contraction in clustering is formation of fibronectin (FN) fibrillar matrix. We found that in serum or LPA but not in PDGF or basal medium, cells organized FN (both serum and cellular) into a fibrillar, detergent-insoluble matrix. Cell clusters developed concomitant with FN matrix formation. FN fibrils accumulated beneath cells and along the borders of cell clusters in regions of cell-matrix tension. Blocking Rho kinase or myosin II activity prevented FN matrix assembly and cell clustering. Using siRNA silencing and function-blocking antibodies and peptides, we found that cell clustering and FN matrix assembly required α5β1 integrins and fibronectin. Cells were still able to exert contractile force and compact the collagen matrix under the latter conditions, which showed that contraction was not sufficient for cell clustering to occur. Our findings provide new insights into how procontractile (serum/LPA) and promigratory (PDGF) growth factor environments can differentially regulate FN matrix assembly by fibroblasts interacting with collagen matrices and thereby influence mesenchymal cell morphogenetic behavior under physiologic circumstances such as wound repair, morphogenesis and malignancy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.