Evidence map›Paper›PMID 23116708›Full record

ReviewMethods (San Diego, Calif.)2013

Mass spectrometry-based identification of S-nitrosocysteine in vivo using organic mercury assisted enrichment.

Paschalis-Thomas Doulias, Karthik Raju, Jennifer L Greene, Margarita Tenopoulou, Harry Ischiropoulos

Open access · greenAbstract readReview
In one paragraph

Review in Methods (San Diego, Calif.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 25 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Paschalis-Thomas DouliasDepartment of Pediatrics, Children's Hospital of Philadelphia Research Institute, Philadelphia, PA 19104, USA.
Karthik Raju
Jennifer L Greene
Margarita Tenopoulou
Harry Ischiropoulos
Children's Hospital of Philadelphia · USUniversity of Pennsylvania · US

Funding

Reactive Species in Vascular Disease: Mechanisms of InjuryR01HL054926 · NHLBI · UNIVERSITY OF PENNSYLVANIA · PI ISCHIROPOULOS, HARRY · 1997 to 2023
$7.7M
Hybrid Triple Quadrupole Mass Spectrometer for Quantitative Mass Spectrometric ApS10RR025668 · NCRR · CHILDREN'S HOSP OF PHILADELPHIA · PI ISCHIROPOULOS, HARRY · 2010 to 2010
$500k
NCRR NIH HHS S10 RR025668NHLBI NIH HHS R01 HL054926
6 · The paper itself

Abstract

Protein S-nitrosylation is considered as one of the molecular mechanisms by which nitric oxide regulates signaling events and protein function. The present review presents an updated method which allows for the site-specific detection of S-nitrosylated proteins in vivo. The method is based on enrichment of S-nitrosylated proteins or peptides using organomercury compounds followed by LC-MS/MS detection. Technical aspects for determining the reaction and binding efficiency of the mercury resin that assists enrichment of S-nitrosylated proteins are presented and discussed. In addition, emphasis is given to the specificity of the method by providing technical details for the generation of four chemically distinct negative controls. Finally it is provided an overview of the key steps for generation and evaluation of mass spectrometry derived data.

Indexed as

AnimalsChromatography, AffinityCysteineHumansMuramidasePeptide FragmentsProtein BindingProtein Processing, Post-TranslationalProteomeS-NitrosothiolsTandem Mass SpectrometryCysteineMuramidasePeptide FragmentsProteomeS-nitrosocysteineS-Nitrosothiols2-(N-morpholino)ethanesulfonic acid4-(2-hydroxyethyl)-1-piperazineethanesulfonic acidacetonitrileACNCysteine modificationdiethylene-triamine-pentaacetic acidDTPAHepesMass spectrometryMESMMTSNitric oxideProtein S-nitrosylationSDSS-methyl methanethiosulfonatesodium dodecyl sulfate

Identifiers

PMID23116708
PMCPMC3573241
OpenAlexW1984168130

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.