ArticlePloS one2012
Visualization of endothelial actin cytoskeleton in the mouse retina.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Silactins and Structural Diversity of Biosilica in Sponges.Biomimetics (Basel, Switzerland) · 2024Article
- Compromised Blood-Brain Barrier Junctions Enhance Melanoma Cell Intercalation and Extravasation.Cancers · 2023Article
- Contractile apparatus in CNS capillary pericytes.Neurophotonics · 2022Review
- miR-155 regulates physiological angiogenesis but an miR-155-rich microenvironment disrupts the process by promoting unproductive endothelial sprouting.Cellular and molecular life sciences : CMLS · 2022Article
- Endothelial junctional membrane protrusions serve as hotspots for neutrophil transmigration.eLife · 2021Article
- Overexpression of Lifeact-GFP Disrupts F-Actin Organization in Cardiomyocytes and Impairs Cardiac Function.Frontiers in cell and developmental biology · 2021Article
- c-Src controls stability of sprouting blood vessels in the developing retina independently of cell-cell adhesion through focal adhesion assembly.Development (Cambridge, England) · 2020Article
- Dynamic Interplay between Pericytes and Endothelial Cells during Sprouting Angiogenesis.Cells · 2019Article
- Consensus guidelines for the use and interpretation of angiogenesis assays.Angiogenesis · 2018Review
- The RhoGAP Stard13 controls insulin secretion through F-actin remodeling.Molecular metabolism · 2018Article
- Polarized actin and VE-cadherin dynamics regulate junctional remodelling and cell migration during sprouting angiogenesis.Nature communications · 2017Article
- Metabolic Regulation of Angiogenesis in Diabetes and Aging.Physiology (Bethesda, Md.) · 2017Review
- Cell-cell junctional mechanotransduction in endothelial remodeling.Cellular and molecular life sciences : CMLS · 2017Review
- Brain Vascular Imaging Techniques.International journal of molecular sciences · 2016Review
- Flow-induced HDAC1 phosphorylation and nuclear export in angiogenic sprouting.Scientific reports · 2016Article
- F-actin-rich contractile endothelial pores prevent vascular leakage during leukocyte diapedesis through local RhoA signalling.Nature communications · 2016Article
- Endothelial alpha-parvin controls integrity of developing vasculature and is required for maintenance of cell-cell junctions.Circulation research · 2015Article
- Fascin 1 is dispensable for developmental and tumour angiogenesis.Biology open · 2013Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Angiogenesis requires coordinated changes in cell shape of endothelial cells (ECs), orchestrated by the actin cytoskeleton. The mechanisms that regulate this rearrangement in vivo are poorly understood - largely because of the difficulty to visualize filamentous actin (F-actin) structures with sufficient resolution. Here, we use transgenic mice expressing Lifeact-EGFP to visualize F-actin in ECs. We show that in the retina, Lifeact-EGFP expression is largely restricted to ECs allowing detailed visualization of F-actin in ECs in situ. Lifeact-EGFP labels actin associated with cell-cell junctions, apical and basal membranes and highlights actin-based structures such as filopodia and stress fiber-like cytoplasmic bundles. We also show that in the skin and the skeletal muscle, Lifeact-EGFP is highly expressed in vascular mural cells (vMCs), enabling vMC imaging. In summary, our results indicate that the Lifeact-EGFP transgenic mouse in combination with the postnatal retinal angiogenic model constitutes an excellent system for vascular cell biology research. Our approach is ideally suited to address structural and mechanistic details of angiogenic processes, such as endothelial tip cell migration and fusion, EC polarization or lumen formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.