ArticleCurrent chemical genomics2012
The HaloTag: Improving Soluble Expression and Applications in Protein Functional Analysis.
Article in Current chemical genomics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Scalable phosphotyrosine enrichment with SH2 superbinder enables deep profiling of EGF responses.The EMBO journal · 2026Article
- R2HaPpY: Rapid-robust phosphotyrosine peptide enrichment using HaloTag-Src SH2 pY superbinder.bioRxiv : the preprint server for biology · 2025Article
- Further assessments of ligase LplA-mediated modifications of proteins in vitro and in cellulo.Molecular biology reports · 2022Article
- Nuclear envelope mechanobiology: linking the nuclear structure and function.Nucleus (Austin, Tex.) · 2021Review
- Bright and stable luminescent probes for target engagement profiling in live cells.Nature chemical biology · 2021Article
- Bacterial Vivisection: How Fluorescence-Based Imaging Techniques Shed a Light on the Inner Workings of Bacteria.Microbiology and molecular biology reviews : MMBR · 2020Review
- Visualizing and Manipulating Biological Processes by Using HaloTag and SNAP-Tag Technologies.Chembiochem : a European journal of chemical biology · 2020Review
- A non-fluorescent HaloTag blocker for improved measurement and visualization of protein synthesis in living cells.F1000Research · 2020Article
- Cooperative assembly of a four-molecule signaling complex formed upon T cell antigen receptor activation.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- γ-Neurexin and Frizzled Mediate Parallel Synapse Assembly Pathways Antagonized by Receptor Endocytosis.Neuron · 2018Article
- Nuclear Import ofFrontiers in plant science · 2018Article
- Visualization of Periplasmic and Cytoplasmic Proteins with a Self-Labeling Protein Tag.Journal of bacteriology · 2016Article
- Recombinant expression of a functional myo-inositol-1-phosphate synthase (MIPS) in Mycobacterium smegmatis.The protein journal · 2015Article
- HaloTag technology: a versatile platform for biomedical applications.Bioconjugate chemistry · 2015Review
- Expression, purification and crystallization of the SKICH domain of human TAX1BP1.Acta crystallographica. Section F, Structural biology communications · 2014Article
- Biochemical characterization of a haloalkane dehalogenase DadB from Alcanivorax dieselolei B-5.PloS one · 2014Article
- A genetically encoded fluorescent probe in mammalian cells.Journal of the American Chemical Society · 2013Article
- HaloTag, a Platform Technology for Protein Analysis.Current chemical genomics · 2012Article
- HaloTag® Platform: From Proteomics to Cellular Analysis and Animal Imaging.Current chemical genomics · 2012Article
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2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Technological and methodological advances have been critical for the rapidly evolving field of proteomics. The development of fusion tag systems is essential for purification and analysis of recombinant proteins. The HaloTag is a 34 KDa monomeric protein derived from a bacterial haloalkane dehalogenase. The majority of fusion tags in use today utilize a reversible binding interaction with a specific ligand. The HaloTag system is unique in that it forms a covalent linkage to its chloroalkane ligand. This linkage permits attachment of the HaloTag to a variety of functional reporters, which can be used to label and immobilize recombinant proteins. The success rate for HaloTag expression of soluble proteins is very high and comparable to maltose binding protein (MBP) tag. Furthermore, cleavage of the HaloTag does not result in protein insolubility that often is observed with the MBP tag. In the present report, we describe applications of the HaloTag system in our ongoing investigation of protein-protein interactions of the Y. pestis Type 3 secretion system on a custom protein microarray. We also describe the utilization of affinity purification/mass spectroscopy (AP/MS) to evaluate the utility of the Halo Tag system to characterize DNA binding activity and protein specificity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.