ArticleBiology of reproduction2012
Porcine sperm bind to specific 6-sialylated biantennary glycans to form the oviduct reservoir.
Article in Biology of reproduction, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
29 citing papers in PubMed, 76 citations in OpenAlex.
- From reservoir to rendezvous: the journey of sperm through the oviduct.Reproduction, fertility, and development · 2026Review
- Let the female tract choose: finding the sperm to enhanceAnimal reproduction · 2026Article
- Sperm-female interactions in the pig oviduct, a key for insemination success?The Journal of reproduction and development · 2026Review
- Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct.Scientific reports · 2025Article
- Oviduct epithelium interactions: roles in sperm selection and embryo quality.Animal reproduction · 2025Article
- Article
- Sperm in the Mammalian Female Reproductive Tract: Surfing Through the Tract to Try to Beat the Odds.Annual review of animal biosciences · 2024Review
- An oviduct glycan increases sperm lifespan by diminishing the production of ubiquinone and reactive oxygen species†.Biology of reproduction · 2023Article
- Sperm selection by the oviduct: perspectives for male fertility and assisted reproductive technologies†.Biology of reproduction · 2023Article
- Modification of Morphology and Glycan Pattern of the Oviductal Epithelium of BaboonAnimals : an open access journal from MDPI · 2022Article
- Review
- Hyperactivation is sufficient to release porcine sperm from immobilized oviduct glycans.Scientific reports · 2022Article
- Diversity of sialic acids and sialoglycoproteins in gametes and at fertilization.Frontiers in cell and developmental biology · 2022Review
- Sperm migration, selection, survival, and fertilizing ability in the mammalian oviduct†.Biology of reproduction · 2021Review
- Progesterone induces porcine sperm release from oviduct glycans in a proteasome-dependent manner.Reproduction (Cambridge, England) · 2021Article
- Serum N-Glycome Diversity in Teleost and Chondrostrean Fishes.Frontiers in molecular biosciences · 2021Article
- The role of semen and seminal plasma in inducing large-scale genomic changes in the female porcine peri-ovulatory tract.Scientific reports · 2020Article
- A Higher Abundance of O-Linked Glycans Confers a Selective Advantage to High Fertile Buffalo Spermatozoa for Immune-Evasion From Neutrophils.Frontiers in immunology · 2020Article
- Article
- Release of Porcine Sperm from Oviduct Cells is Stimulated by Progesterone and Requires CatSper.Scientific reports · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
After mating, many female mammals store a subpopulation of sperm in the lower portion of the oviduct, forming a reservoir. The reservoir lengthens sperm lifespan, regulates sperm capacitation, controls polyspermy, and selects normal sperm. It is believed that sperm bind to glycans on the oviduct epithelium to form the reservoir, but the specific adhesion molecules that retain sperm are unclear. Herein, using a glycan array to test 377 glycans for their ability to bind porcine sperm, we found two glycan motifs in common among all glycans with sperm-binding ability: the Lewis X trisaccharide and biantennary structures containing a mannose core with 6-sialylated lactosamine at one or more termini. Binding to both motifs was specific; isomers of each motif did not bind sperm. Further work focused on sialylated lactosamine. Sialylated lactosamine was found abundantly on the apical side of epithelial cells collected from the oviduct isthmus, among N-linked and O-linked glycans. Sialylated lactosamine bound to the head of sperm, the region that interacts with the oviduct epithelium. After capacitation, sperm lost affinity for sialylated lactosamine. Receptor modification may contribute to release from the reservoir so that sperm can move to the site of fertilization. Sialylated lactosamine was required for sperm to bind oviduct cells. Simbucus nigra agglutinin or an antibody specific to sialylated lactosamine with a preference for Neu5Acalpha2-6Gal rather than Neu5Acalpha2-3Gal reduced sperm binding to oviduct isthmic cells, as did occupying putative receptors on sperm with sialylated biantennary glycans. These results demonstrate that sperm binding to oviduct 6-sialylated biantennary glycans is necessary for normal adhesion to the oviduct.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.