ArticleAmerican journal of physiology. Regulatory, integrative and comparative physiology2012
Greater insulin-mediated Akt phosphorylation concomitant with heterogeneous effects on phosphorylation of Akt substrates in soleus of calorie-restricted rats.
Article in American journal of physiology. Regulatory, integrative and comparative physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- Intramuscular MAPK signaling following high volume and high intensity resistance exercise protocols in trained men.European journal of applied physiology · 2016Trial
- Residual force enhancement following hindlimb unloading and exercise prehabilitation.Journal of muscle research and cell motility · 2025Article
- Independent and combined effects of calorie restriction and AICAR on glucose uptake and insulin signaling in skeletal muscles from 24-month-old female and male rats.Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme · 2024Article
- Inhibition of Akt2 phosphorylation abolishes the calorie restriction-induced improvement in insulin-stimulated glucose uptake by rat soleus muscle.Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme · 2016Article
- Insulin Signaling and Glucose Uptake in the Soleus Muscle of 30-Month-Old Rats After Calorie Restriction With or Without Acute Exercise.The journals of gerontology. Series A, Biological sciences and medical sciences · 2016Article
- Intramuscular anabolic signaling and endocrine response following high volume and high intensity resistance exercise protocols in trained men.Physiological reports · 2015Article
- Association between myosin heavy chain protein isoforms and intramuscular anabolic signaling following resistance exercise in trained men.Physiological reports · 2015Article
- Heterogeneous effects of calorie restriction on in vivo glucose uptake and insulin signaling of individual rat skeletal muscles.PloS one · 2014Article
- Greater filamin C, GSK3α, and GSK3β serine phosphorylation in insulin-stimulated isolated skeletal muscles of calorie restricted 24 month-old rats.Mechanisms of ageing and developmentArticle
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Akt is a serine/threonine kinase that plays a key role in numerous cellular functions including metabolism, growth, protein synthesis, apoptosis, and cell proliferation. The most consistent and robust effect of moderate calorie restriction (CR; ~60% of ad libitum, AL, food consumption) on insulin signaling in rodent muscle has been enhanced insulin-induced phosphorylation of Akt (pAkt). However, there is limited knowledge regarding the mechanism for this enhancement and its consequences in predominantly slow-twitch muscle. Accordingly, in soleus muscle of 9-mo-old rats, we analyzed the effect of CR and insulin on important signaling events that are proximal to Akt activation including: pIR(Tyr1162/1163), pIRS1(Tyr), pIRS1(Ser312), IRS1-associated phosphatidylinositol 3-kinase activity, or pPTEN(Ser380). In addition, we analyzed the effect of CR and insulin on Akt substrates that have established or putative roles in glucose metabolism, cellular growth, maintenance of muscle structure, or protein synthesis including pGSK3α(Ser21), pGSK3β(Ser9), pTSC2(Ser939), pP70S6K(Thr412), pAS160(Thr642), and pFLNc(Ser2213). The current study demonstrated that the CR-induced increase in pAkt in isolated soleus muscles from 9-mo-old rats can occur without concomitant enhancement of several important insulin signaling events that are proximal to Akt activation. These results suggest that the greater pAkt in the soleus muscles from CR rats was attributable to an alternative mechanism. We also observed that the effects of CR were not uniform for phosphorylation of six insulin-regulated Akt substrates in the soleus. The differential response in phosphorylation by Akt substrates likely has important implications for explaining the complex effect of CR diverse cellular functions.
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