ArticleThe Journal of antimicrobial chemotherapy2013
In vitro emergence of rifampicin resistance in Propionibacterium acnes and molecular characterization of mutations in the rpoB gene.
Article in The Journal of antimicrobial chemotherapy, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 33 citations in OpenAlex.
- Synergistic Antimicrobial Activity of Vancomycin, Ceftriaxone, and Gentamicin AgainstAntibiotics (Basel, Switzerland) · 2025Article
- Genomic characteristics and phylogenetic relationships of Cutibacterium acnes breast milk isolates.BMC microbiology · 2025Article
- Cutibacterium namnetense osteosynthetic cervical spine infections: experience with two cases.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Article
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- The Impact of Surgical Strategy and Rifampin on Treatment Outcome in Cutibacterium Periprosthetic Joint Infections.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2021Article
- Prosthetic Shoulder Joint Infection byAntibiotics (Basel, Switzerland) · 2021Article
- Transferable Multidrug-Resistance Plasmid Carrying a Novel Macrolide-Clindamycin Resistance Gene,Antimicrobial agents and chemotherapy · 2020Article
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- Rifampin-ResistantJournal of bone and joint infection · 2018Article
- Finegoldia magna Isolated from Orthopedic Joint Implant-Associated Infections.Journal of clinical microbiology · 2017Article
- Draft Genome Sequences of Four Propionibacterium acnes Strains Isolated from Implant-Related Infections.Genome announcements · 2016Article
- Draft Genome Sequence of Highly Rifampin-Resistant Propionibacterium namnetense NTS 31307302T Isolated from a Patient with a Bone Infection.Genome announcements · 2016Article
- Which is the best treatment for prosthetic joint infections due to Propionibacterium acnes: need for further biofilm in vitro and experimental foreign-body in vivo studies?Acta orthopaedica · 2016Article
- Immunoproteomic Identification of In Vivo-Produced Propionibacterium acnes Proteins in a Rabbit Biofilm Infection Model.Clinical and vaccine immunology : CVI · 2015Article
- Propionibacterium acnes: from commensal to opportunistic biofilm-associated implant pathogen.Clinical microbiology reviews · 2014Review
- Propionibacterium acnes infection after shoulder surgery.International journal of shoulder surgeryReview
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3 authors at 2 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
objectivesActivity of rifampicin against Propionibacterium acnes biofilms was recently demonstrated, but rifampicin resistance has not yet been described in this organism. We investigated the in vitro emergence of rifampicin resistance in P. acnes and characterized its molecular background.
methodsP. acnes ATCC 11827 was used (MIC 0.007 mg/L). The mutation rate was determined by inoculation of 10(9) cfu of P. acnes on rifampicin-containing agar plates incubated anaerobically for 7 days. Progressive emergence of resistance was studied by serial exposure to increasing concentrations of rifampicin in 72 h cycles using a low (10(6) cfu/mL) and high (10(8) cfu/mL) inoculum. The stability of resistance was determined after three subcultures of rifampicin-resistant isolates on rifampicin-free agar. For resistant mutants, the whole rpoB gene was amplified, sequenced and compared with a P. acnes reference sequence (NC006085).
resultsP. acnes growth was observed on rifampicin-containing plates with mutation rates of 2 ± 1 cfu × 10(-9) (4096× MIC) and 12 ± 5 cfu × 10(-9) (4 × MIC). High-level rifampicin resistance emerged progressively after 4 (high inoculum) and 13 (low inoculum) cycles. In rifampicin-resistant isolates, the MIC remained >32 mg/L after three subcultures. Mutations were detected in clusters I (amino acids 418-444) and II (amino acids 471-486) of the rpoB gene after sequence alignment with a Staphylococcus aureus reference sequence (CAA45512). The five following substitutions were found: His-437 → Tyr, Ser-442 → Leu, Leu-444 → Ser, Ile-483 → Val and Ser-485 → Leu.
conclusionThe rifampicin MIC increased from highly susceptible to highly resistant values. The resistance remained stable and was associated with mutations in the rpoB gene. To our knowledge, this is the first report of the emergence of rifampicin resistance in P. acnes.
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