ArticleAmerican journal of physiology. Cell physiology2013
Effects of ACE inhibition and ANG II stimulation on renal Na-Cl cotransporter distribution, phosphorylation, and membrane complex properties.
Article in American journal of physiology. Cell physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 41 citations in OpenAlex.
- Age-Related Adaptations in Renal Tubular Function in Female Rats.Acta physiologica (Oxford, England) · 2026Article
- Article
- Role of SPAK-NKCC1 signaling cascade in the choroid plexus blood-CSF barrier damage after stroke.Journal of neuroinflammation · 2022Article
- Coordinate adaptations of skeletal muscle and kidney to maintain extracellular [KAmerican journal of physiology. Cell physiology · 2020Article
- Impact of angiotensin II-mediated stimulation of sodium transporters in the nephron assessed by computational modeling.American journal of physiology. Renal physiology · 2019Article
- Consequences of SPAK inactivation on Hyperkalemic Hypertension caused by WNK1 mutations: evidence for differential roles of WNK1 and WNK4.Scientific reports · 2018Article
- The thiazide sensitive sodium chloride co-transporter NCC is modulated by site-specific ubiquitylation.Scientific reports · 2017Article
- Cardiovascular benefits associated with higher dietary KAmerican journal of physiology. Endocrinology and metabolism · 2017Review
- Potassium Homeostasis: The Knowns, the Unknowns, and the Health Benefits.Physiology (Bethesda, Md.) · 2017Review
- Potassium Supplementation Prevents Sodium Chloride Cotransporter Stimulation During Angiotensin II Hypertension.Hypertension (Dallas, Tex. : 1979) · 2016Article
- The sodium chloride cotransporter (NCC) and epithelial sodium channel (ENaC) associate.The Biochemical journal · 2016Article
- The mineralocorticoid receptor (MR) regulates ENaC but not NCC in mice with random MR deletion.Pflugers Archiv : European journal of physiology · 2016Article
- Short-term nonpressor angiotensin II infusion stimulates sodium transporters in proximal tubule and distal nephron.Physiological reports · 2015Article
- Maintaining Balance Under Pressure: Integrated Regulation of Renal Transporters During Hypertension.Hypertension (Dallas, Tex. : 1979) · 2015Review
- Renal NCC is unchanged in the midpregnant rat and decreased in the late pregnant rat despite avid renal Na+ retention.American journal of physiology. Renal physiology · 2015Article
- SPAK-mediated NCC regulation in response to low-K+ diet.American journal of physiology. Renal physiology · 2015Article
- Mechanisms of angiotensin II stimulation of NCC are time-dependent in mDCT15 cells.American journal of physiology. Renal physiology · 2015Article
- Considerations when quantitating protein abundance by immunoblot.American journal of physiology. Cell physiology · 2015Review
- Dietary potassium and the renal control of salt balance and blood pressure.Pflugers Archiv : European journal of physiology · 2015Review
- Distal convoluted tubule.Comprehensive Physiology · 2015Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
Abstract
The renal distal tubule Na-Cl cotransporter (NCC) reabsorbs <10% of the filtered Na(+) but is a key control point for blood pressure regulation by angiotensin II (ANG II), angiotensin-converting enzyme inhibitors (ACEI), and thiazide diuretics. This study aimed to determine whether NCC phosphorylation (NCCp) was regulated by acute (20-30 min) treatment with the ACEI captopril (12 μg/min × 20 min) or by a sub-pressor dose of ANG II (20 ng·kg(-1)·min(-1)) in Inactin-anesthetized rats. By immuno-EM, NCCp was detected exclusively in or adjacent to apical plama membranes (APM) in controls and after ACEI or ANG II treatment, while NCC total was detected in both APM and subapical cytoplasmic vesicles (SCV) in all conditions. In renal homogenates, neither ACEI nor ANG II treatment altered NCCp abundance, assayed by immunoblot. However, by density gradient fractionation we identified a pool of low-density APM in which NCCp decreased 50% in response to captopril and was restored during ANG II infusion, and another pool of higher-density APM that responded reciprocally, indicative of regulated redistribution between two APM pools. In both pools, NCCp was preferentially localized to Triton-soluble membranes. Blue Native gel electrophoresis established that APM NCCp localized to ~700 kDa complexes (containing γ-adducin) while unphosphorylated NCC in intracellular membranes primarily localized to ~400 kDa complexes: there was no evidence for native monomeric or dimeric NCC or NCCp. In summary, this study demonstrates that phosphorylated NCC, localized to multimeric complexes in the APM, redistributes in a regulated manner within the APM in response to ACEI and ANG II.
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