ArticleAntimicrobial agents and chemotherapy2013
Rapid isolation and susceptibility testing of Leptospira spp. using a new solid medium, LVW agar.
Article in Antimicrobial agents and chemotherapy, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 42 citations in OpenAlex.
- Leptospira and Leptospirosis: A Review of Species Classifications, Genomes, Morphological Structures, Antimicrobial Resistances, Transmissions, and Clinical Manifestations.Current microbiology · 2026Review
- Antibacterial Activity of CrudeActa medica Philippina · 2024Article
- Serological and molecular epidemiology of leptospirosis and the role of dogs as sentinel for human infection in Nigeria.Heliyon · 2022Article
- Towards routine employment of computational tools for antimicrobial resistance determination via high-throughput sequencing.Briefings in bioinformatics · 2022Article
- Analysis of human clinical and environmental Leptospira to elucidate the eco-epidemiology of leptospirosis in Yaeyama, subtropical Japan.PLoS neglected tropical diseases · 2022Article
- Survival ofThe American journal of tropical medicine and hygiene · 2020Article
- Isolation and propagation of leptospires at 37 °C directly from the mammalian host.Scientific reports · 2020Article
- Leptospiral Culture without 5'-Fluorouracil Revealed ImprovedInternational journal of environmental research and public health · 2020Article
- Antimicrobial Susceptibility Testing ofThe American journal of tropical medicine and hygiene · 2019Article
- Whole cell matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for identification of Leptospira spp. in Thailand and Lao PDR.PLoS neglected tropical diseases · 2019Article
- Antimicrobial Resistance inMicrobiology spectrum · 2018Review
- Quality controls for antimicrobial disk diffusion testing on Leptospira Vanaporn Wuthiekanun agar.Transactions of the Royal Society of Tropical Medicine and Hygiene · 2016Article
- Leptospirosis: risk factors and management challenges in developing countries.Research and reports in tropical medicine · 2016Review
- Antimicrobial Disk Susceptibility Testing of Leptospira spp. Using Leptospira Vanaporn Wuthiekanun (LVW) Agar.The American journal of tropical medicine and hygiene · 2015Article
- Current and past strategies for bacterial culture in clinical microbiology.Clinical microbiology reviews · 2015Review
- Antibiotic Efficacy in Eliminating Leptospiruria in California Sea Lions (Aquatic mammals · 2015Article
- Maintenance of leptospira species in leptospira Vanaporn Wuthiekanun agar.Journal of clinical microbiology · 2014Article
- Leptospira species in floodwater during the 2011 floods in the Bangkok Metropolitan Region, Thailand.The American journal of tropical medicine and hygiene · 2013Article
- Prospective evaluation of three rapid diagnostic tests for diagnosis of human leptospirosis.PLoS neglected tropical diseases · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
Abstract
Pathogenic Leptospira spp., the causative agents of leptospirosis, are slow-growing Gram-negative spirochetes. Isolation of Leptospira from clinical samples and testing of antimicrobial susceptibility are difficult and time-consuming. Here, we describe the development of a new solid medium that facilitates more-rapid growth of Leptospira spp. and the use of this medium to evaluate the Etest's performance in determining antimicrobial MICs to drugs in common use for leptospirosis. The medium was developed by evaluating the effects of numerous factors on the growth rate of Leptospira interrogans strain NR-20157. These included the type of base agar, the concentration of rabbit serum (RS), and the concentration and duration of CO(2) incubation during the initial period of culture. The highest growth rate of NR-20157 was achieved using a Noble agar base supplemented with 10% RS (named LVW agar), with an initial incubation at 30°C in 5% CO(2) for 2 days prior to continuous culture in air at 30°C. These conditions were used to develop the Etest for three species, L. interrogans (NR-20161), L. kirschnerii (NR-20327), and L. borgpetersenii (NR-20151). The MICs were read on day 7 for all samples. The Etest was then performed on 109 isolates of pathogenic Leptospira spp. The MIC(90) values for penicillin G, doxycycline, cefotaxime, ceftriaxone, and chloramphenicol were 0.64 units/ml and 0.19, 0.047, 0.5, and 2 μg/ml, respectively. The use of LVW agar, which enables rapid growth, isolation of single colonies, and simple antimicrobial susceptibility testing for Leptospira spp., provides an opportunity for new areas of fundamental and applied research.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.