ArticleMicrobial cell factories2012
An automated workflow for enhancing microbial bioprocess optimization on a novel microbioreactor platform.
Article in Microbial cell factories, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 137 citations in OpenAlex.
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- Early bioprocess development using Komagataella phaffii: application scenarios for the microTOM mini bioreactor system.Journal of biological engineering · 2026Article
- Droplet based microfluidic and miniaturization at high-throughput level to screening heterologous secondary metabolites in microbes: A critical review.Biotechnology letters · 2025Review
- Microtiter Plate Cultivation Systems Enable Chemically Diverse Metabolic Footprints During Bacterial Natural Product Discovery.Biotechnology and bioengineering · 2025Article
- Automation of a Capillary-Wave Microbioreactor Platform to Enhance Phage Sensitivity Screen Efficiency.Engineering in life sciences · 2025Article
- Design of fully synthetic signal peptide library and its use for enhanced secretory production of recombinant proteins in Corynebacterium glutamicum.Microbial cell factories · 2024Article
- Combinatorial optimization of pathway, process and media for the production of p-coumaric acid by Saccharomyces cerevisiae.Microbial biotechnology · 2024Article
- From frozen cell bank to product assay: high-throughput strain characterisation for autonomous Design-Build-Test-Learn cycles.Microbial cell factories · 2023Article
- An automated workflow for multi-omics screening of microbial model organisms.NPJ systems biology and applications · 2023Article
- Black glucose-releasing silicon elastomer rings for fed-batch operation allow measurement of the oxygen transfer rate from the top and optical signals from the bottom for each well of a microtiter plate.BMC biotechnology · 2023Article
- Assessing the capabilities of 2D fluorescence monitoring in microtiter plates with data-driven modeling for secondary substrate limitation experiments of Hansenula polymorpha.Journal of biological engineering · 2023Article
- Control of parallelized bioreactors II: probabilistic quantification of carboxylic acid reductase activity for bioprocess optimization.Bioprocess and biosystems engineering · 2022Article
- Microsensor in Microbioreactors: Full Bioprocess Characterization in a Novel Capillary-Wave Microbioreactor.Biosensors · 2022Article
- Strain Development in Microalgal Biotechnology-Random Mutagenesis Techniques.Life (Basel, Switzerland) · 2022Review
- Metabolic Footprinting of Microbial Systems Based on Comprehensive In Silico Predictions of MS/MS Relevant Data.Metabolites · 2022Article
- bletl - A Python package for integrating BioLector microcultivation devices in the Design-Build-Test-Learn cycle.Engineering in life sciences · 2022Article
- Rapid screening of high expressing Escherichia coli colonies using a novel dicistronic-autoinducible system.Microbial cell factories · 2021Article
- Automation assisted anaerobic phenotyping for metabolic engineering.Microbial cell factories · 2021Article
- Development of a defined medium for3 Biotech · 2021Article
- Need for speed: evaluation of dilute and shoot-mass spectrometry for accelerated metabolic phenotyping in bioprocess development.Analytical and bioanalytical chemistry · 2021Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHigh-throughput methods are widely-used for strain screening effectively resulting in binary information regarding high or low productivity. Nevertheless achieving quantitative and scalable parameters for fast bioprocess development is much more challenging, especially for heterologous protein production. Here, the nature of the foreign protein makes it impossible to predict the, e.g. best expression construct, secretion signal peptide, inductor concentration, induction time, temperature and substrate feed rate in fed-batch operation to name only a few. Therefore, a high number of systematic experiments are necessary to elucidate the best conditions for heterologous expression of each new protein of interest.
resultsTo increase the throughput in bioprocess development, we used a microtiter plate based cultivation system (Biolector) which was fully integrated into a liquid-handling platform enclosed in laminar airflow housing. This automated cultivation platform was used for optimization of the secretory production of a cutinase from Fusarium solani pisi with Corynebacterium glutamicum. The online monitoring of biomass, dissolved oxygen and pH in each of the microtiter plate wells enables to trigger sampling or dosing events with the pipetting robot used for a reliable selection of best performing cutinase producers. In addition to this, further automated methods like media optimization and induction profiling were developed and validated. All biological and bioprocess parameters were exclusively optimized at microtiter plate scale and showed perfect scalable results to 1 L and 20 L stirred tank bioreactor scale.
conclusionsThe optimization of heterologous protein expression in microbial systems currently requires extensive testing of biological and bioprocess engineering parameters. This can be efficiently boosted by using a microtiter plate cultivation setup embedded into a liquid-handling system, providing more throughput by parallelization and automation. Due to improved statistics by replicate cultivations, automated downstream analysis, and scalable process information, this setup has superior performance compared to standard microtiter plate cultivation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.