ArticleIranian journal of public health2012
Use of Single-enzyme PCR-restriction Digestion Barcode Targeting the Internal Transcribed Spacers (ITS rDNA) to Identify Dermatophyte Species.
Article in Iranian journal of public health, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Update on the epidemiology of dermatophytosis in Isfahan, Iran over the past decade.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025Article
- Molecular Diagnostics and Control of Zoonotic Dermatophytosis: First Detection ofAnimals : an open access journal from MDPI · 2025Article
- Addressing filamentous fungi-related onychomycosis in the era of antifungal resistance: assessment ofCurrent medical mycology · 2025Article
- [Conventional and molecular diagnostics in onychomycosis-part 2 : Molecular identification of causative dermatophytes by polymerase chain reaction and sequence analysis of the internal transcribed spacer region of ribosomal DNA].Dermatologie (Heidelberg, Germany) · 2024Article
- Dermatophytosis caused by Nannizzia nana (Microsporum nanum): a comprehensive review on a novel pathogen.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Review
- Comparison of in vitro activities of newer triazoles and classic antifungal agents against dermatophyte species isolated from Iranian University Hospitals: a multi-central study.Annals of clinical microbiology and antimicrobials · 2023Article
- Caspian Sea Mycosands: The Variety and Abundance of Medically Important Fungi in Beach Sand and Water.International journal of environmental research and public health · 2022Article
- First report of tinea corporis caused by Trichophyton quinckeanum in Iran and its antifungal susceptibility profile.Current medical mycology · 2022Article
- Anti-dermatophytic activity of cold atmospheric plasma against Trichophyton rubrum via affecting fungal growth, morphology, drug susceptibility and HSP90 gene expression.Scientific reports · 2022Article
- A simple multiplex polymerase chain reaction assay for rapid identification of the common pathogenic dermatophytes: Trichophyton interdigitale, Trichophyton rubrum, and Epidermophyton floccosum.Current medical mycology · 2021Article
- Diversity of Geophilic Dermatophytes Species in the Soils of Iran; The Significant Preponderance ofJournal of fungi (Basel, Switzerland) · 2021Article
- Article
- Epidemiology of dermatophytosis in northeastern Iran; A subtropical region.Current medical mycology · 2019Article
- Article
- Distribution of Species of Dermatophyte Among Patients at a Dermatology Centre of Nghean Province, Vietnam, 2015-2016.Mycopathologia · 2017Article
- Mycological aspects of onychomycosis in Khuzestan Province, Iran: A shift from dermatophytes towards yeasts.Current medical mycology · 2017Article
- Dermatophytosis due to Microsporum incurvatum: Notification and Identification of a Neglected Pathogenic Species.Mycopathologia · 2016Article
- Molecular Characterization and In Vitro Antifungal Susceptibility of 316 Clinical Isolates of Dermatophytes in Iran.Mycopathologia · 2016Article
- Use of Restriction Fragment Length Polymorphism to Rapidly Identify Dermatophyte Species Related to Dermatophytosis.Jundishapur journal of microbiology · 2015Article
- Restriction analysis of β-tubulin gene for differentiation of the common pathogenic dermatophytes.Journal of clinical laboratory analysis · 2014Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDermatophytes are the most common causative agents of superficial mycoses. Species identification of these fungi is important from therapeutic and epidemiological point of wive. Traditional approaches for identification of dermatophytes at the species level, relying on macroscopic and microscopic features of the colonies, usually are time-consuming and unreliable in many circumstances. Recently a broad varieties of rapid and accurate DNA-based techniques were successfuly utilized for species delineation of dermatophytes.
methodsThe ITS1-5.8S-ITS2 region of rDNA from various reference strains of dermatophyte species were amplified using the universal fungal primers ITS1 and ITS4.The PCR products were digested by a single restriction enzyme, MvaI. The enzyme was evaluated in both in silico and practical PCR-RFLP assay to find the exact differentiating restriction profiles for each species. To validate the standardized PCR-RFLP system, all tested strains were subjected to sequencing and sequence analysis.
resultsThe obtained RFLP patterns were specific for many species including T. interdigitale, T. rubrum, T. violaceum, M. persicolor, M. audouinii, M. nanum (A. obtusum) and E. floccosum but were similar for some closely related species such as M. canis / M. ferrugineum. Sequencing of the ITS1-5.8S-ITS2 fragment from all type strains affirmed the RFLP findings.
conclusionIt was practically revealed that the ITS-PCR followed by MvaI-RFLP is a useful and reliable schema for identification and differentiation of several pathogenic species and can be used for rapid screening of even closely related species of dermatophytes in clinical and epidemiological settings.
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