ArticleIranian journal of public health2011
Detection of Borrelia persica Infection in Ornithodoros tholozani Using PCR Targeting rrs Gene and Xenodiagnosis.
Article in Iranian journal of public health, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- Diversity of Rickettsiales inPathogens (Basel, Switzerland) · 2022Article
- Study on Hard and Soft Ticks of Domestic and Wild Animals in Western Iran.Journal of arthropod-borne diseases · 2022Article
- Ornithodoros (Pavlovskyella) ticks associated with a Rickettsia sp. in Pakistan.Parasites & vectors · 2022Article
- An integrated overview of the bacterial flora composition of Hyalomma anatolicum, the main vector of CCHF.PLoS neglected tropical diseases · 2021Article
- Rapid clearance of Borrelia burgdorferi from the blood circulation.Parasites & vectors · 2020Article
- Development of A Loop-Mediated Isothermal Amplification (LAMP) Assay for Detection of Relapsing Fever Borreliae.Journal of arthropod-borne diseases · 2020Article
- A study of ticks and tick-borne livestock pathogens in Pakistan.PLoS neglected tropical diseases · 2017Article
- Borrelia persica Infection in Immunocompetent Mice--A New Tool to Study the Infection Kinetics In Vivo.PLoS neglected tropical diseases · 2016Article
- Vectors of Crimean Congo Hemorrhagic Fever Virus in Iran.Journal of arthropod-borne diseases · 2015Review
- Study on Presence of Borrelia persica in Soft Ticks in Western Iran.Iranian journal of arthropod-borne diseases · 2010Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRelapsing fever caused by Borrelia persica, is an acute tick-borne disease which is transmitted by soft ticks of Ornithodoros tholozani to human.
methodsValue of PCR and xenodiagnosis for detection of B. persica in O. tholozani ticks was compared. Sixty-four Borrelia-free ticks were fed on infected guinea pigs and used for the experiments. For xenodiagnosis, a group of 32 ticks in subsequent blood meal were fed on sterile guinea pigs and the indication of B. persica in the animal blood was tested 5-14 days later by dark-field microscopy. For PCR, all 64 ticks were subjected to PCR against B. persica rrs gene (16S-rDNA). Also sensitivity of PCR in terms of minimum detectable number of spirochetes as well as the effects of tick sex and post digestion was tested.
resultsPCR revealed B. persica DNA in 98.4% ticks, in which B. persica were found in 25.0% by xenodiagnosis. PCR was enough sensitive to give positive results for DNA of 1 spirochete. PCR success rates were similar for male or female ticks. Course of time did not affect the efficacy of PCR and similar results were observed for ticks of immediately fed, semi- or completely gravid or completely digested blood ones.
conclusionOur results indicate that due to very low specificity and time consuming, xenodiagnosis is not a useful method whereas PCR method has advantages for study the Borrelia prevalence in ticks.
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Identifiers
23113113PMC3481739W2156078072What OpenQuestion holds
Registered trials
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