ArticlePloS one2012
Stability of p53 homologs.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Congenital heart disease missense mutations in the TBX5 DNA-binding domain alter thermal stability and DNA-binding affinity.G3 (Bethesda, Md.) · 2025Article
- TP53: the unluckiest of genes?Cell death and differentiation · 2025Review
- Nucleo-cytoplasmic environment modulates spatiotemporal p53 phase separation.Science advances · 2024Article
- Structural basis of p53 inactivation by cavity-creating cancer mutations and its implications for the development of mutant p53 reactivators.Cell death & disease · 2024Article
- Evolutionary history of the p53 family DNA-binding domain: insights from an Alvinella pompejana homolog.Cell death & disease · 2022Article
- Functional interplay among thiol-based redox signaling, metabolism, and ferroptosis unveiled by a genetic variant ofProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Targeting Cavity-Creating p53 Cancer Mutations with Small-Molecule Stabilizers: the Y220X Paradigm.ACS chemical biology · 2020Article
- Simulations of mutant p53 DNA binding domains reveal a novel druggable pocket.Nucleic acids research · 2019Article
- Structural basis of gene regulation by the Grainyhead/CP2 transcription factor family.Nucleic acids research · 2018Article
- Post-translational regulation of p53 function through 20S proteasome-mediated cleavage.Cell death and differentiation · 2017Article
- Effects of ligand binding on the stability of aldo-keto reductases: Implications for stabilizer or destabilizer chaperones.Protein science : a publication of the Protein Society · 2016Article
- Intrinsic aggregation propensity of the p63 and p73 TI domains correlates with p53R175H interaction and suggests further significance of aggregation events in the p53 family.Cell death and differentiation · 2016Article
- Aggregation tendencies in the p53 family are modulated by backbone hydrogen bonds.Scientific reports · 2016Article
- Article
- Propagation of aggregated p53: Cross-reaction and coaggregation vs. seeding.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Conformational stability and catalytic activity of PTEN variants linked to cancers and autism spectrum disorders.Biochemistry · 2015Article
- Evolution of p53 transactivation specificity through the lens of a yeast-based functional assay.PloS one · 2015Article
- ∆N-P63α and TA-P63α exhibit intrinsic differences in transactivation specificities that depend on distinct features of DNA target sites.Oncotarget · 2014Article
- Quantification of excluded volume effects on the folding landscape of Pseudomonas aeruginosa apoazurin in vitro.Biophysical journal · 2013Article
- Designing disorder: Tales of the unexpected tails.Intrinsically disordered proteinsReview
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Authors and funding
4 authors.
Funding
Abstract
Most proteins have not evolved for maximal thermal stability. Some are only marginally stable, as for example, the DNA-binding domains of p53 and its homologs, whose kinetic and thermodynamic stabilities are strongly correlated. Here, we applied high-throughput methods using a real-time PCR thermocycler to study the stability of several full-length orthologs and paralogs of the p53 family of transcription factors, which have diverse functions, ranging from tumour suppression to control of developmental processes. From isothermal denaturation fluorimetry and differential scanning fluorimetry, we found that full-length proteins showed the same correlation between kinetic and thermodynamic stability as their isolated DNA-binding domains. The stabilities of the full-length p53 orthologs were marginal and correlated with the temperature of their organism, paralleling the stability of the isolated DNA-binding domains. Additionally, the paralogs p63 and p73 were significantly more stable and long-lived than p53. The short half-life of p53 orthologs and the greater persistence of the paralogs may be biologically relevant.
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