ArticlePloS one2012
One-step Agrobacterium mediated transformation of eight genes essential for rhizobium symbiotic signaling using the novel binary vector system pHUGE.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Spatiotemporal dynamics of ethylene biosynthesis shape infection and nodule initiation in Medicago truncatula.The Plant cell · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Synthetic crassulacean acid metabolism (SynCAM) for improving water-use efficiency in plants.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Inactivation of Three Stacked Genes of Cytosolic Peroxiredoxins by Genome Editing.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Efficient Gene Stacking in Rice Using the GAANTRY System.Rice (New York, N.Y.) · 2021Article
- Gene Assembly in Agrobacterium via Nucleic Acid Transfer Using Recombinase Technology (GAANTRY).Methods in molecular biology (Clifton, N.J.) · 2021Article
- Genome-wide analysis of the TCP transcription factor genes in five legume genomes and their response to salt and drought stresses.Functional & integrative genomics · 2020Article
- Agrobacterium-Mediated Transformation of Chrysanthemum with Artemisinin Biosynthesis Pathway Genes.Plants (Basel, Switzerland) · 2020Article
- A Roadmap toward Engineered Nitrogen-Fixing Nodule Symbiosis.Plant communications · 2020Review
- Plant Biosystems Design Research Roadmap 1.0.Biodesign research · 2020Review
- Are we there yet? The long walk towards the development of efficient symbiotic associations between nitrogen-fixing bacteria and non-leguminous crops.BMC biology · 2019Review
- Comparative genomics of the nonlegumeProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Artificial Agrobacterium tumefaciens strains exhibit diverse mechanisms to repress Xanthomonas oryzae pv. oryzae-induced hypersensitive response and non-host resistance in Nicotiana benthamiana.Molecular plant pathology · 2017Article
- Article
- Transposon based activation tagging in diploid strawberry and monoploid derivatives of potato.Plant cell reports · 2014Article
- pSiM24 is a novel versatile gene expression vector for transient assays as well as stable expression of foreign genes in plants.PloS one · 2014Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Advancement in plant research is becoming impaired by the fact that the transfer of multiple genes is difficult to achieve. Here we present a new binary vector for Agrobacterium tumefaciens mediated transformation, pHUGE-Red, in concert with a cloning strategy suited for the transfer of up to nine genes at once. This vector enables modular cloning of large DNA fragments by employing Gateway technology and contains DsRED1 as visual selection marker. Furthermore, an R/Rs inducible recombination system was included allowing subsequent removal of the selection markers in the newly generated transgenic plants. We show the successful use of pHUGE-Red by transferring eight genes essential for Medicago truncatula to establish a symbiosis with rhizobia bacteria as one 74 kb T-DNA into four non-leguminous species; strawberry, poplar, tomato and tobacco. We provide evidence that all transgenes are expressed in the root tissue of the non-legumes. Visual control during the transformation process and subsequent marker gene removal makes the pHUGE-Red vector an excellent tool for the efficient transfer of multiple genes.
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