ArticlePloS one2012
Identification, expression and activity analyses of five novel duck beta-defensins.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
- Pathogenicity and innate immune responses induced by fowl adenovirus serotype 8b in specific pathogen-free chicken.Poultry science · 2023Article
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- Comparative analysis of early immune responses induced by two strains of Newcastle disease virus in chickens.MicrobiologyOpen · 2019Article
- Effects ofPeerJ · 2019Article
- Induction of Avian β-Defensin 2 Is Possibly Mediated by the p38 MAPK Signal Pathway in Chicken Embryo Fibroblasts After Newcastle Disease Virus Infection.Frontiers in microbiology · 2018Article
- Virologic and Immunologic Characteristics in Mature Ducks with Acute Duck Hepatitis A Virus 1 Infection.Frontiers in immunology · 2017Article
- Infection of Goose with Genotype VIId Newcastle Disease Virus of Goose Origin Elicits Strong Immune Responses at Early Stage.Frontiers in microbiology · 2016Article
- Expression of Immune-Related Genes of Ducks Infected with Avian Pathogenic Escherichia coli (APEC).Frontiers in microbiology · 2016Article
- Differential modulation of avian β-defensin and Toll-like receptor expression in chickens infected with infectious bronchitis virus.Applied microbiology and biotechnology · 2015Article
- Host Avian Beta-Defensin and Toll-Like Receptor Responses of Pigeons following Infection with Pigeon Paramyxovirus Type 1.Applied and environmental microbiology · 2015Article
- Antimicrobial activities of chicken β-defensin (4 and 10) peptides against pathogenic bacteria and fungi.Frontiers in cellular and infection microbiology · 2015Article
- Avian antimicrobial host defense peptides: from biology to therapeutic applications.Pharmaceuticals (Basel, Switzerland) · 2014Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the current study, five novel avian β-defensins (AvBDs) were identified and characterized in tissues from Peking ducks (Anas platyrhynchos). The nucleotide sequences of these cDNAs comprised 198 bp, 182 bp, 201 bp, 204 bp, and 168 bp, and encoded 65, 60, 66, 67, and 55 amino acids, respectively. Homology, characterization and comparison of these genes with AvBD from other avian species confirmed that they were Apl_AvBD1, 3, 5, 6, and 16. Recombinant AvBDs were produced and purified by expressing these genes in Escherichia coli. In addition, peptides were synthesized according to the respective AvBD sequences. Investigation of the antibacterial activity of the Apl_AvBDs showed that all of them exhibited antibacterial activity against all 12 bacteria investigated (P<0.05 or P<0.01). In addition, the antibacterial activity of all of the AvBDs against M. tetragenus and P. multocida decreased significantly in the presence of 150 mM NaCl (P<0.01). None of the AvBDs showed hemolytic activity. Consistent with their broad-spectrum antibacterial activity, the five novel Apl_AvBDs inhibited replication of duck hepatitis virus (DHV) in vitro significantly (P<0.05). The mRNA expression of all five Apl_AvBD in most tissues, including immune organs and the liver, was upregulated in response to DHV infection at different time points. These findings provide evidence that these defensins activate the immune response to combat microbial infection.
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