ArticlePloS one2012
The metagenome-derived enzymes LipS and LipT increase the diversity of known lipases.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 82 citations in OpenAlex.
- Structural and Biochemical Insights into Bis(2-hydroxyethyl) Terephthalate Degrading Carboxylesterase Isolated from Psychrotrophic BacteriumInternational journal of molecular sciences · 2023Article
- Molecular characterization of transesterification activity of novel lipase family I.1.Bioscience reports · 2022Article
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- Metagenomic Screening for Lipolytic Genes Reveals an Ecology-Clustered Distribution Pattern.Frontiers in microbiology · 2022Article
- Characterization of a Novel Esterase Est33 From an Antarctic Bacterium: A Representative of a New Esterase Family.Frontiers in microbiology · 2022Article
- Enhanced Thermostability of D-Psicose 3-Epimerase fromInternational journal of molecular sciences · 2021Article
- A multi-omic screening approach for the discovery of thermoactive glycoside hydrolases.Extremophiles : life under extreme conditions · 2021Article
- Study on a Novel Cold-Active and Halotolerant Monoacylglycerol Lipase Widespread in Marine Bacteria Reveals a New Group of Bacterial Monoacylglycerol Lipases Containing Unusual C(A/S)HSMG Catalytic Motifs.Frontiers in microbiology · 2020Article
- Crystal structure of PMGL2 esterase from the hormone-sensitive lipase family with GCSAG motif around the catalytic serine.PloS one · 2020Article
- Biodegradation aspects of ibuprofen and identification of ibuprofen-degrading microbiota in an immobilized cell bioreactor.Environmental science and pollution research international · 2019Article
- Pseudomonas aeruginosa esterase PA2949, a bacterial homolog of the human membrane esterase ABHD6: expression, purification and crystallization.Acta crystallographica. Section F, Structural biology communications · 2019Article
- A proposed update for the classification and description of bacterial lipolytic enzymes.PeerJ · 2019Article
- Activity-based protein profiling as a robust method for enzyme identification and screening in extremophilic Archaea.Nature communications · 2017Article
- Metagenomic discovery of novel enzymes and biosurfactants in a slaughterhouse biofilm microbial community.Scientific reports · 2016Article
- Metagenomics of Thermophiles with a Focus on Discovery of Novel Thermozymes.Frontiers in microbiology · 2016Review
- Fast and reliable production, purification and characterization of heat-stable, bifunctional enzyme chimeras.AMB Express · 2015Article
- Thermus thermophilus as a Source of Thermostable Lipolytic Enzymes.Microorganisms · 2015Review
- Characterization of Novel Family IV Esterase and Family I.3 Lipase from an Oil-Polluted Mud Flat Metagenome.Molecular biotechnology · 2015Article
- Immobilization and characterization of a new regioselective and enantioselective lipase obtained from a metagenomic library.PloS one · 2015Article
- Identification of novel esterase-active enzymes from hot environments by use of the host bacterium Thermus thermophilus.Frontiers in microbiology · 2015Article
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Authors and funding
18 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Triacylglycerol lipases (EC 3.1.1.3) catalyze both hydrolysis and synthesis reactions with a broad spectrum of substrates rendering them especially suitable for many biotechnological applications. Most lipases used today originate from mesophilic organisms and are susceptible to thermal denaturation whereas only few possess high thermotolerance. Here, we report on the identification and characterization of two novel thermostable bacterial lipases identified by functional metagenomic screenings. Metagenomic libraries were constructed from enrichment cultures maintained at 65 to 75 °C and screened resulting in the identification of initially 10 clones with lipolytic activities. Subsequently, two ORFs were identified encoding lipases, LipS and LipT. Comparative sequence analyses suggested that both enzymes are members of novel lipase families. LipS is a 30.2 kDa protein and revealed a half-life of 48 h at 70 °C. The lipT gene encoded for a multimeric enzyme with a half-life of 3 h at 70 °C. LipS had an optimum temperature at 70 °C and LipT at 75 °C. Both enzymes catalyzed hydrolysis of long-chain (C(12) and C(14)) fatty acid esters and additionally hydrolyzed a number of industry-relevant substrates. LipS was highly specific for (R)-ibuprofen-phenyl ester with an enantiomeric excess (ee) of 99%. Furthermore, LipS was able to synthesize 1-propyl laurate and 1-tetradecyl myristate at 70 °C with rates similar to those of the lipase CalB from Candida antarctica. LipS represents the first example of a thermostable metagenome-derived lipase with significant synthesis activities. Its X-ray structure was solved with a resolution of 1.99 Å revealing an unusually compact lid structure.
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