ArticleSensors (Basel, Switzerland)2012
Label-free electrochemical diagnosis of viral antigens with genetically engineered fusion protein.
Article in Sensors (Basel, Switzerland), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 21 citations in OpenAlex.
- Biomedical applications of solid-binding peptides and proteins.Materials today. Bio · 2023Review
- Reagentless electrochemical biosensors through incorporation of unnatural amino acids on the protein structure.Biosensors & bioelectronics · 2022Article
- The perspectives of biomarker-based electrochemical immunosensors, artificial intelligence and the Internet of Medical Things toward COVID-19 diagnosis and management.Materials today. Chemistry · 2021Review
- Current methods and prospects of coronavirus detection.Talanta · 2021Review
- Ultrasensitive detection of pathogenic viruses with electrochemical biosensor: State of the art.Biosensors & bioelectronics · 2020Review
- A Highly Sensitive Detection System based on Proximity-dependent Hybridization with Computer-aided Affinity Maturation of a scFv Antibody.Scientific reports · 2018Article
- Site-selective orientated immobilization of antibodies and conjugates for immunodiagnostics development.Methods (San Diego, Calif.) · 2017Review
- Nanoyeast and Other Cell Envelope Compositions for Protein Studies and Biosensor Applications.ACS applied materials & interfaces · 2016Review
- Label-free electrochemical detection of an Entamoeba histolytica antigen using cell-free yeast-scFv probes.Chemical communications (Cambridge, England) · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a simple electrochemical biosensing strategy for the label-free diagnosis of hepatitis B virus (HBV) on a gold electrode surface. Gold-binding polypeptide (GBP) fused with single-chain antibody (ScFv) against HBV surface antigen (HBsAg), in forms of genetically engineered protein, was utilized. This GBP-ScFv fusion protein can directly bind onto the gold substrate with the strong binding affinity between the GBP and the gold surface, while the recognition site orients toward the sample for target binding at the same time. Furthermore, this one-step immobilization strategy greatly simplifies a fabrication process without any chemical modification as well as maintaining activity of biological recognition elements. This system allows specific immobilization of proteins and sensitive detection of targets, which were verified by surface plasmon resonance analysis and successfully applied to electrochemical cyclic voltammetry and impedance spectroscopy upto 0.14 ng/mL HBsAg.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.