ArticleProceedings of the National Academy of Sciences of the United States of America2012
MscS-Like10 is a stretch-activated ion channel from Arabidopsis thaliana with a preference for anions.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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71 citing papers in PubMed, 141 citations in OpenAlex.
- Phytoacoustics: sound perception, mechanosensing, and biological responses to sound in plants.Plant signaling & behavior · 2026Review
- The bacterial mechanosensitive channel MscM gates through concerted changes in its transmembrane and cytoplasmic domains.Nature communications · 2026Article
- MscM uses a novel gating mechanism for bacterial mechanosensitive channels.bioRxiv : the preprint server for biology · 2026Article
- Mechanisms of Induction of Stimulus-Specific Systemic Responses of Photosynthesis in Wheat Plants.International journal of molecular sciences · 2025Article
- MSL10 is a high-sensitivity mechanosensor in the tactile sense of the Venus flytrap.Nature communications · 2025Article
- Lipid interactions and gating hysteresis suggest a physiological role for mechanosensitive channel YnaI.Nature communications · 2025Article
- A mechanosensitive ion channel controls touch-triggered stigma movement through manipulation of calcium signature in Torenia.Nature communications · 2025Article
- Dynamics of homeostats: the basis of electrical, chemical, hydraulic, pH and calcium signaling in plants.Quantitative plant biology · 2025Review
- Analysis of the Mechanisms Underlying the Specificity of the Variation Potential Induced by Different Stimuli.Plants (Basel, Switzerland) · 2024Article
- Channels of Evolution: Unveiling Evolutionary Patterns in Diatom CaPlants (Basel, Switzerland) · 2024Article
- Ion Changes and Signaling under Salt Stress in Wheat and Other Important Crops.Plants (Basel, Switzerland) · 2023Review
- Mechanically-primed voltage-gated proton channels from angiosperm plants.Nature communications · 2023Article
- Review
- Open structure and gating of the Arabidopsis mechanosensitive ion channel MSL10.Nature communications · 2023Article
- Mechanosensitive ion channels MSL8, MSL9, and MSL10 have environmentally sensitive intrinsically disordered regions with distinct biophysical characteristics in vitro.Plant direct · 2023Article
- Mechanosensitive membrane proteins: Usual and unusual suspects in mediating mechanotransduction.The Journal of general physiology · 2023Article
- Molecular response and evolution of plant anion transport systems to abiotic stress.Plant molecular biology · 2022Review
- Article
- Root osmotic sensing from local perception to systemic responses.Stress biology · 2022Review
- OzTracs: Optical Osmolality Reporters Engineered from Mechanosensitive Ion Channels.Biomolecules · 2022Article
11 more citing papers are in PubMed but not listed here.
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2 authors at 1 institution in 1 country.
Funding
Abstract
Like many other organisms, plants are capable of sensing and responding to mechanical stimuli such as touch, osmotic pressure, and gravity. One mechanism for the perception of force is the activation of mechanosensitive (or stretch-activated) ion channels, and a number of mechanosensitive channel activities have been described in plant membranes. Based on their homology to the bacterial mechanosensitive channel MscS, the 10 MscS-Like (MSL) proteins of Arabidopsis thaliana have been hypothesized to form mechanosensitive channels in plant cell and organelle membranes. However, definitive proof that MSLs form mechanosensitive channels has been lacking. Here we used single-channel patch clamp electrophysiology to show that MSL10 is capable of providing a MS channel activity when heterologously expressed in Xenopus laevis oocytes. This channel had a conductance of ∼100 pS, consistent with the hypothesis that it underlies an activity previously observed in the plasma membrane of plant root cells. We found that MSL10 formed a channel with a moderate preference for anions, which was modulated by strongly positive and negative membrane potentials, and was reversibly inhibited by gadolinium, a known inhibitor of mechanosensitive channels. MSL10 demonstrated asymmetric activation/inactivation kinetics, with the channel closing at substantially lower tensions than channel opening. The electrophysiological characterization of MSL10 reported here provides insight into the evolution of structure and function of this important family of proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.