ArticlePloS one2012
The τCstF-64 polyadenylation protein controls genome expression in testis.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 26 citations in OpenAlex.
- Deciphering the impact of genetic variation on human polyadenylation using APARENT2.Genome biology · 2022Article
- First Infertile Case withMolecular syndromology · 2020Article
- A missense mutation in the CSTF2 gene that impairs the function of the RNA recognition motif and causes defects in 3' end processing is associated with intellectual disability in humans.Nucleic acids research · 2020Article
- Tissue-specific mechanisms of alternative polyadenylation: Testis, brain, and beyond (2018 update).Wiley interdisciplinary reviews. RNA · 2019Review
- The structural basis of CstF-77 modulation of cleavage and polyadenylation through stimulation of CstF-64 activity.Nucleic acids research · 2018Article
- Cstf2t Regulates expression of histones and histone-like proteins in male germ cells.Andrology · 2018Article
- Nonsense in the testis: multiple roles for nonsense-mediated decay revealed in male reproduction.Biology of reproduction · 2017Review
- TauCstF-64 Mediates Correct mRNA Polyadenylation and Splicing of Activator and Repressor Isoforms of the Cyclic AMP-Responsive Element Modulator (CREM) in Mouse Testis.Biology of reproduction · 2016Article
- Alternative cleavage and polyadenylation in spermatogenesis connects chromatin regulation with post-transcriptional control.BMC biology · 2016Article
- The Cstf2t Polyadenylation Gene Plays a Sex-Specific Role in Learning Behaviors in Mice.PloS one · 2016Article
- Systematic profiling of poly(A)+ transcripts modulated by core 3' end processing and splicing factors reveals regulatory rules of alternative cleavage and polyadenylation.PLoS genetics · 2015Article
- Specific Tandem 3'UTR Patterns and Gene Expression Profiles in Mouse Thy1+ Germline Stem Cells.PloS one · 2015Article
- CstF-64 is necessary for endoderm differentiation resulting in cardiomyocyte defects.Stem cell research · 2014Article
- New links between mRNA polyadenylation and diverse nuclear pathways.Molecules and cells · 2014Review
- CstF-64 supports pluripotency and regulates cell cycle progression in embryonic stem cells through histone 3' end processing.Nucleic acids research · 2014Article
- Delineating the structural blueprint of the pre-mRNA 3'-end processing machinery.Molecular and cellular biology · 2014Review
- High-throughput sequencing of RNA isolated by cross-linking and immunoprecipitation (HITS-CLIP) to determine sites of binding of CstF-64 on nascent RNAs.Methods in molecular biology (Clifton, N.J.) · 2014Article
- mRNA 3'end processing: A tale of the tail reaches the clinic.EMBO molecular medicine · 2014Review
- Overlapping and distinct functions of CstF64 and CstF64τ in mammalian mRNA 3' processing.RNA (New York, N.Y.) · 2013Article
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
The τCstF-64 polyadenylation protein (gene symbol Cstf2t) is a testis-expressed orthologue of CstF-64. Mice in which Cstf2t was knocked out had a phenotype that was only detected in meiotic and postmeiotic male germ cells, giving us the opportunity to examine CstF-64 function in an isolated developmental system. We performed massively parallel clonally amplified sequencing of cDNAs from testes of wild type and Cstf2t(-/-) mice. These results revealed that loss of τCstF-64 resulted in large-scale changes in patterns of genome expression. We determined that there was a significant overrepresentation of RNAs from introns and intergenic regions in testes of Cstf2t(-/-) mice, and a concomitant use of more distal polyadenylation sites. We observed this effect particularly in intronless small genes, many of which are expressed retroposons that likely co-evolved with τCstF-64. Finally, we observed overexpression of long interspersed nuclear element (LINE) sequences in Cstf2t(-/-) testes. These results suggest that τCstF-64 plays a role in 3' end determination and transcription termination for a large range of germ cell-expressed genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.