ArticlePLoS biology2012
The molecular basis for recognition of CD1d/α-galactosylceramide by a human non-Vα24 T cell receptor.
Article in PLoS biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
- Ligand-induced segregation from large cell-surface phosphatases is a critical step in γδ TCR triggering.Cell reports · 2024Article
- Unedited allogeneic iNKT cells show extended persistence in MHC-mismatched canine recipients.Cell reports. Medicine · 2023Article
- Identification of a broad lipid repertoire associated to the endothelial cell protein C receptor (EPCR).Scientific reports · 2022Article
- Structural plasticity in I-AFrontiers in immunology · 2022Article
- Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition).European journal of immunology · 2021Review
- Article
- Novel Molecular Insights into Human Lipid-Mediated T Cell Immunity.International journal of molecular sciences · 2021Review
- Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).European journal of immunology · 2019Article
- Development of α-GalCer Analogues with an α-Fluorocarbonyl Moiety as Th2-Selective Ligands of CD1d.ACS medicinal chemistry letters · 2019Article
- The lipid transfer protein Saposin B does not directly bind CD1d for lipid antigen loading.Wellcome open research · 2019Article
- Human TRAV1-2-negative MR1-restricted T cells detect S. pyogenes and alternatives to MAIT riboflavin-based antigens.Nature communications · 2016Article
- Atypical natural killer T-cell receptor recognition of CD1d-lipid antigens.Nature communications · 2016Article
- The Extended Family of CD1d-Restricted NKT Cells: Sifting through a Mixed Bag of TCRs, Antigens, and Functions.Frontiers in immunology · 2015Review
- Potent neutralizing anti-CD1d antibody reduces lung cytokine release in primate asthma model.mAbs · 2015Article
- The CD1 size problem: lipid antigens, ligands, and scaffolds.Cellular and molecular life sciences : CMLS · 2014Review
- Design and Evaluation of ω-Hydroxy Fatty Acids Containing α-GalCer Analogues for CD1d-Mediated NKT Cell Activation.ACS medicinal chemistry letters · 2014Article
- MAIT recognition of a stimulatory bacterial antigen bound to MR1.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Invariant natural killer T cells: an innate activation scheme linked to diverse effector functions.Nature reviews. Immunology · 2013Review
- Recognition of CD1d-restricted antigens by natural killer T cells.Nature reviews. Immunology · 2012Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
CD1d-mediated presentation of glycolipid antigens to T cells is capable of initiating powerful immune responses that can have a beneficial impact on many diseases. Molecular analyses have recently detailed the lipid antigen recognition strategies utilized by the invariant Vα24-Jα18 TCR rearrangements of iNKT cells, which comprise a subset of the human CD1d-restricted T cell population. In contrast, little is known about how lipid antigens are recognized by functionally distinct CD1d-restricted T cells bearing different TCRα chain rearrangements. Here we present crystallographic and biophysical analyses of α-galactosylceramide (α-GalCer) recognition by a human CD1d-restricted TCR that utilizes a Vα3.1-Jα18 rearrangement and displays a more restricted specificity for α-linked glycolipids than that of iNKT TCRs. Despite having sequence divergence in the CDR1α and CDR2α loops, this TCR employs a convergent recognition strategy to engage CD1d/αGalCer, with a binding affinity (∼2 µM) almost identical to that of an iNKT TCR used in this study. The CDR3α loop, similar in sequence to iNKT-TCRs, engages CD1d/αGalCer in a similar position as that seen with iNKT-TCRs, however fewer actual contacts are made. Instead, the CDR1α loop contributes important contacts to CD1d/αGalCer, with an emphasis on the 4'OH of the galactose headgroup. This is consistent with the inability of Vα24- T cells to respond to α-glucosylceramide, which differs from αGalCer in the position of the 4'OH. These data illustrate how fine specificity for a lipid containing α-linked galactose is achieved by a TCR structurally distinct from that of iNKT cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.