Isolation and characterization of 13 new polymorphic microsatellite markers in the Phaseolus vulgaris L. (Common Bean) genome.

Aihua Wang, Yi Ding, Zhenhua Hu, Chufa Lin, Shuzhen Wang, Bingcai Wang, Hongyuan Zhang, Guolin Zhou

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Article in International journal of molecular sciences, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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8 authors.

Aihua WangHubei Provincial Engineering Research Center of Vegetable Seeds and Seedlings, Wuhan Vegetable Research Institute, Wuhan Academy of Agricultural Science & Technology, Wuhan 430345, China.

Yi DingState Key Laboratory of Hybrid Rice, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, China.

Zhenhua HuHubei Provincial Engineering Research Center of Vegetable Seeds and Seedlings, Wuhan Vegetable Research Institute, Wuhan Academy of Agricultural Science & Technology, Wuhan 430345, China.

Chufa LinHubei Provincial Engineering Research Center of Vegetable Seeds and Seedlings, Wuhan Vegetable Research Institute, Wuhan Academy of Agricultural Science & Technology, Wuhan 430345, China.

Shuzhen WangState Key Laboratory of Hybrid Rice, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, China.

Bingcai WangHubei Provincial Engineering Research Center of Vegetable Seeds and Seedlings, Wuhan Vegetable Research Institute, Wuhan Academy of Agricultural Science & Technology, Wuhan 430345, China.

Hongyuan ZhangState Key Laboratory of Hybrid Rice, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, China.

Guolin ZhouHubei Provincial Engineering Research Center of Vegetable Seeds and Seedlings, Wuhan Vegetable Research Institute, Wuhan Academy of Agricultural Science & Technology, Wuhan 430345, China.

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6 · The paper itself

Abstract

In this study, 13 polymorphic microsatellite markers were isolated from the Phaseolus vulgaris L. (common bean) by using the Fast Isolation by AFLP of Sequence COntaining Repeats (FIASCO) protocol. These markers revealed two to seven alleles, with an average of 3.64 alleles per locus. The polymorphic information content (PIC) values ranged from 0.055 to 0.721 over 13 loci, with a mean value of 0.492, and 7 loci having PIC greater than 0.5. The expected heterozygosity (H(E)) and observed heterozygosity (H(O)) levels ranged from 0.057 to 0.814 and from 0.026 to 0.531, respectively. Cross-species amplification of the 13 prime pairs was performed in its related specie of Vigna unguiculata L. Seven out of all these markers showed cross-species transferability. These markers will be useful for future genetic diversity and population genetics studies for this agricultural specie and its related species.

Indexed as

AllelesAmplified Fragment Length Polymorphism AnalysisBase SequenceDNA, PlantGenetic LociGenome, PlantMicrosatellite RepeatsPhaseolusPolymorphism, GeneticSequence Analysis, DNADNA, Plantcross-species transferabilitygenetic diversitymicrosatellite markersPhaseolus vulgaris L.

Identifiers

PMID23109846

DOI10.3390/ijms130911188

PMCPMC3472738

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