Evidence map›Paper›PMID 23109720›Full record

ArticleThe Journal of heredity

Development and application of camelid molecular cytogenetic tools.

Felipe Avila, Pranab J Das, Michelle Kutzler, Elaine Owens, Polina Perelman, Jiri Rubes, Miroslav Hornak, Warren E Johnson, Terje Raudsepp

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of heredity. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.6field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 24 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Chromosome-Level Alpaca Reference GenomeFrontiers in genetics · 2019
    Article
  7. Comparative FISH-Mapping ofFrontiers in genetics · 2019
    Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. A cytogenetic and comparative map of camelid chromosome 36 and the minute in alpacas.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Felipe AvilaFrom the Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX 77843 (Avila, Das, and Raudsepp); Department of Animal Sciences, College of Agricultural Sciences, Oregon State University, Corvallis, OR 97331 (Kutzler); Department of Veterinary Pathobiology, Texas A&M University, College Station, TX 77843 (Owens); Laboratory of Genomic Diversity, National Cancer Institute, Frederick, MD 21702 (Perelman and Johnson); Laboratory of Cytogenetics of Animals, Institute of Molecular and Cellular Biology, Novosibirsk, Russia (Perelman); and Veterinary Research Institute, Brno, Czech Republic (Rubes and Hornak).
Pranab J DasFrom the Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX 77843 (Avila, Das, and Raudsepp); Department of Animal Sciences, College of Agricultural Sciences, Oregon State University, Corvallis, OR 97331 (Kutzler); Department of Veterinary Pathobiology, Texas A&M University, College Station, TX 77843 (Owens); Laboratory of Genomic Diversity, National Cancer Institute, Frederick, MD 21702 (Perelman and Johnson); Laboratory of Cytogenetics of Animals, Institute of Molecular and Cellular Biology, Novosibirsk, Russia (Perelman); and Veterinary Research Institute, Brno, Czech Republic (Rubes and Hornak).
Michelle KutzlerFrom the Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX 77843 (Avila, Das, and Raudsepp); Department of Animal Sciences, College of Agricultural Sciences, Oregon State University, Corvallis, OR 97331 (Kutzler); Department of Veterinary Pathobiology, Texas A&M University, College Station, TX 77843 (Owens); Laboratory of Genomic Diversity, National Cancer Institute, Frederick, MD 21702 (Perelman and Johnson); Laboratory of Cytogenetics of Animals, Institute of Molecular and Cellular Biology, Novosibirsk, Russia (Perelman); and Veterinary Research Institute, Brno, Czech Republic (Rubes and Hornak).
Elaine OwensFrom the Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX 77843 (Avila, Das, and Raudsepp); Department of Animal Sciences, College of Agricultural Sciences, Oregon State University, Corvallis, OR 97331 (Kutzler); Department of Veterinary Pathobiology, Texas A&M University, College Station, TX 77843 (Owens); Laboratory of Genomic Diversity, National Cancer Institute, Frederick, MD 21702 (Perelman and Johnson); Laboratory of Cytogenetics of Animals, Institute of Molecular and Cellular Biology, Novosibirsk, Russia (Perelman); and Veterinary Research Institute, Brno, Czech Republic (Rubes and Hornak).
Polina PerelmanFrom the Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX 77843 (Avila, Das, and Raudsepp); Department of Animal Sciences, College of Agricultural Sciences, Oregon State University, Corvallis, OR 97331 (Kutzler); Department of Veterinary Pathobiology, Texas A&M University, College Station, TX 77843 (Owens); Laboratory of Genomic Diversity, National Cancer Institute, Frederick, MD 21702 (Perelman and Johnson); Laboratory of Cytogenetics of Animals, Institute of Molecular and Cellular Biology, Novosibirsk, Russia (Perelman); and Veterinary Research Institute, Brno, Czech Republic (Rubes and Hornak).
Jiri RubesFrom the Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX 77843 (Avila, Das, and Raudsepp); Department of Animal Sciences, College of Agricultural Sciences, Oregon State University, Corvallis, OR 97331 (Kutzler); Department of Veterinary Pathobiology, Texas A&M University, College Station, TX 77843 (Owens); Laboratory of Genomic Diversity, National Cancer Institute, Frederick, MD 21702 (Perelman and Johnson); Laboratory of Cytogenetics of Animals, Institute of Molecular and Cellular Biology, Novosibirsk, Russia (Perelman); and Veterinary Research Institute, Brno, Czech Republic (Rubes and Hornak).
Miroslav HornakFrom the Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX 77843 (Avila, Das, and Raudsepp); Department of Animal Sciences, College of Agricultural Sciences, Oregon State University, Corvallis, OR 97331 (Kutzler); Department of Veterinary Pathobiology, Texas A&M University, College Station, TX 77843 (Owens); Laboratory of Genomic Diversity, National Cancer Institute, Frederick, MD 21702 (Perelman and Johnson); Laboratory of Cytogenetics of Animals, Institute of Molecular and Cellular Biology, Novosibirsk, Russia (Perelman); and Veterinary Research Institute, Brno, Czech Republic (Rubes and Hornak).
Warren E JohnsonFrom the Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX 77843 (Avila, Das, and Raudsepp); Department of Animal Sciences, College of Agricultural Sciences, Oregon State University, Corvallis, OR 97331 (Kutzler); Department of Veterinary Pathobiology, Texas A&M University, College Station, TX 77843 (Owens); Laboratory of Genomic Diversity, National Cancer Institute, Frederick, MD 21702 (Perelman and Johnson); Laboratory of Cytogenetics of Animals, Institute of Molecular and Cellular Biology, Novosibirsk, Russia (Perelman); and Veterinary Research Institute, Brno, Czech Republic (Rubes and Hornak).
Terje RaudseppFrom the Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX 77843 (Avila, Das, and Raudsepp); Department of Animal Sciences, College of Agricultural Sciences, Oregon State University, Corvallis, OR 97331 (Kutzler); Department of Veterinary Pathobiology, Texas A&M University, College Station, TX 77843 (Owens); Laboratory of Genomic Diversity, National Cancer Institute, Frederick, MD 21702 (Perelman and Johnson); Laboratory of Cytogenetics of Animals, Institute of Molecular and Cellular Biology, Novosibirsk, Russia (Perelman); and Veterinary Research Institute, Brno, Czech Republic (Rubes and Hornak). traudsepp@cvm.tamu.edu.
Oregon State University · USInstitute of Molecular and Cell Biology · RUTexas A&M University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cytogenetic chromosome maps offer molecular tools for genome analysis and clinical cytogenetics and are of particular importance for species with difficult karyotypes, such as camelids (2n = 74). Building on the available human-camel zoo-fluorescence in situ hybridization (FISH) data, we developed the first cytogenetic map for the alpaca (Lama pacos, LPA) genome by isolating and identifying 151 alpaca bacterial artificial chromosome (BAC) clones corresponding to 44 specific genes. The genes were mapped by FISH to 31 alpaca autosomes and the sex chromosomes; 11 chromosomes had 2 markers, which were ordered by dual-color FISH. The STS gene mapped to Xpter/Ypter, demarcating the pseudoautosomal region, whereas no markers were assigned to chromosomes 14, 21, 22, 28, and 36. The chromosome-specific markers were applied in clinical cytogenetics to identify LPA20, the major histocompatibility complex (MHC)-carrying chromosome, as a part of an autosomal translocation in a sterile male llama (Lama glama, LGL; 2n = 73,XY). FISH with LPAX BACs and LPA36 paints, as well as comparative genomic hybridization, were also used to investigate the origin of the minute chromosome, an abnormally small LPA36 in infertile female alpacas. This collection of cytogenetically mapped markers represents a new tool for camelid clinical cytogenetics and has applications for the improvement of the alpaca genome map and sequence assembly.

Indexed as

Genetic MarkersAnimalsCamelids, New WorldChromosome MappingChromosomes, Artificial, BacterialComparative Genomic HybridizationFemaleIn Situ Hybridization, FluorescenceKaryotypingMaleSex ChromosomesGenetic MarkersalpacaBAC librarycytogeneticsFISHminute chromosometranslocation

Identifiers

PMID23109720
PMCPMC4207859
OpenAlexW2157383610

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.