ArticleSmall (Weinheim an der Bergstrasse, Germany)2013
Microfluidic investigation of BDNF-enhanced neural stem cell chemotaxis in CXCL12 gradients.
Article in Small (Weinheim an der Bergstrasse, Germany), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- "Next-Generation Cord Blood Expansion: Bridging the Cell Dose Gap with Bioengineered Niches and Clinical Breakthroughs".Stem cell reviews and reports · 2026Review
- Targeting Ischemic Stroke with Neural Stem Cells: Insights into Endogenous Repair Mechanisms, Biomaterial-Based Delivery, and Exosome Therapies.Molecular neurobiology · 2025Review
- CXCR4 as a novel target in immunology: moving away from typical antagonists.Future drug discovery · 2022Review
- Neuroregeneration and functional recovery after stroke: advancing neural stem cell therapy toward clinical application.Neural regeneration research · 2021Article
- Potential Mechanisms and Perspectives in Ischemic Stroke Treatment Using Stem Cell Therapies.Frontiers in cell and developmental biology · 2021Review
- A Concentration Gradients Tunable Generator with Adjustable Position of the Acoustically Oscillating Bubbles.Micromachines · 2020Article
- VEGF Upregulates EGFR Expression to Stimulate Chemotactic Behaviors in the rMC-1 Model of Müller Glia.Brain sciences · 2020Article
- Flow-Free Microfluidic Device for Quantifying Chemotaxis in Spermatozoa.Analytical chemistry · 2020Article
- Microfluidic three-dimensional cell culture of stem cells for high-throughput analysis.World journal of stem cells · 2019Review
- Label-free cell sorting strategies via biophysical and biochemical gradients.Journal of orthopaedic translation · 2019Review
- Prenatal Environment That Affects Neuronal Migration.Frontiers in cell and developmental biology · 2019Review
- The brain, sirtuins, and ageing.Nature reviews. Neuroscience · 2017Review
- Microfluidic systems for stem cell-based neural tissue engineering.Lab on a chip · 2016Review
- Study on chemotaxis and chemokinesis of bone marrow-derived mesenchymal stem cells in hydrogel-based 3D microfluidic devices.Biomaterials research · 2016Article
- Effect of interferon-β1b on CXCR4-dependent chemotaxis in T cells from multiple sclerosis patients.Clinical and experimental immunology · 2015Article
- Microfluidics: reframing biological enquiry.Nature reviews. Molecular cell biology · 2015Review
- Opportunities and challenges: stem cell-based therapy for the treatment of ischemic stroke.CNS neuroscience & therapeutics · 2015Review
- BDNF-TrkB axis regulates migration of the lateral line primordium and modulates the maintenance of mechanoreceptor progenitors.PloS one · 2015Article
- Inducing chemotactic and haptotactic cues in microfluidic devices for three-dimensional in vitro assays.Biomicrofluidics · 2014Article
- Targeting CXCR7/ACKR3 as a therapeutic strategy to promote remyelination in the adult central nervous system.The Journal of experimental medicine · 2014Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
In vivo studies have suggested that gradients of CXCL12 (aka stromal cell-derived factor 1α) may be critical for neural stem cell (NSC) migration during brain development and neural tissue regeneration. However, traditional in vitro chemotaxis tools are limited by unstable concentration gradients and the inability to decouple cell migration directionality and speed. These limitations have restricted the reproducible and quantitative analysis of neuronal migration, which is required for mechanism-based studies. Using a microfluidic gradient generator, nestin and Sox-2 positive human embryonic NSC chemotaxis is quantified within a linear and stable CXCL12 gradient. While untreated NSCs are not able to chemotax within CXCL12 gradients, pre-treatment of the cells with brain-derived neurotrophic factor (BDNF) results in significant chemotactic, directional migration. BDNF pre-treatment has no effect on cell migration speed, which averages about 1 μm min(-1). Quantitative analysis determines that CXCL12 concentrations above 9.0 nM are above the minimum activation threshold, while concentrations below 14.7 nM are below the saturation threshold. Interestingly, although inhibitor studies with AMD 3100 revealed that CXCL12 chemotaxis requires receptor CXCR4 activation, BDNF pre-treatment is found to have no profound effects on the mRNA levels or surface presentation of CXCR4 or the putative CXCR7 scavenger receptor. The microfluidic study of NSC migration within stable chemokine concentration profiles provides quantitative analysis as well as new insight into the migratory mechanism underlying BDNF-induced chemotaxis towards CXCL12.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.