Evidence map›Paper›PMID 23109037›Full record

ArticleBiomedical microdevices2013

Immunomagnetic nanoscreening of circulating tumor cells with a motion controlled microfluidic system.

Yu-Yen Huang, Kazunori Hoshino, Peng Chen, Chun-Hsien Wu, Nancy Lane, Michael Huebschman, Huaying Liu, Konstantin Sokolov, Jonathan W Uhr, Eugene P Frenkel and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical microdevices, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed.

  1. Review
  2. Article
  3. The Applications and Challenges of the Development ofCellular and molecular bioengineering · 2023
    Review
  4. Review
  5. Review
  6. Review
  7. Article
  8. Review
  9. Review
  10. Review
  11. Article
  12. Review
  13. Article
  14. Article
  15. Review
  16. Review
  17. Review
  18. Review
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Yu-Yen HuangDepartment of Biomedical Engineering, The University of Texas at Austin, University Station, C0800, Austin, TX, 78712-0238, USA.
Kazunori HoshinoDepartment of Biomedical Engineering, The University of Texas at Austin, University Station, C0800, Austin, TX, 78712-0238, USA.
Peng ChenDepartment of Biomedical Engineering, The University of Texas at Austin, University Station, C0800, Austin, TX, 78712-0238, USA.
Chun-Hsien WuDepartment of Biomedical Engineering, The University of Texas at Austin, University Station, C0800, Austin, TX, 78712-0238, USA.
Nancy LaneHarold C. Simons Comprehensive Cancer Center of the University of Texas Southwestern, Medical Center, USA, 5323 Harry Hines Boulevard, Dallas, TX, 7390-852, USA.
Michael HuebschmanHarold C. Simons Comprehensive Cancer Center of the University of Texas Southwestern, Medical Center, USA, 5323 Harry Hines Boulevard, Dallas, TX, 7390-852, USA.
Huaying LiuHarold C. Simons Comprehensive Cancer Center of the University of Texas Southwestern, Medical Center, USA, 5323 Harry Hines Boulevard, Dallas, TX, 7390-852, USA.
Konstantin SokolovDepartment of Imaging Physics, Division of Diagnostic Imaging, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX, 77030, USA.
Jonathan W UhrHarold C. Simons Comprehensive Cancer Center of the University of Texas Southwestern, Medical Center, USA, 5323 Harry Hines Boulevard, Dallas, TX, 7390-852, USA.
Eugene P FrenkelHarold C. Simons Comprehensive Cancer Center of the University of Texas Southwestern, Medical Center, USA, 5323 Harry Hines Boulevard, Dallas, TX, 7390-852, USA.
John X J ZhangDepartment of Biomedical Engineering, The University of Texas at Austin, University Station, C0800, Austin, TX, 78712-0238, USA. john.zhang@engr.utexas.edu.

Funding

Detection of CarcinomasR01CA139070 · NCI · UNIVERSITY OF TEXAS AT AUSTIN · PI ZHANG, XIAOJING · 2011 to 2013
$925k
NCI NIH HHS 1R01CA139070NCI NIH HHS R01 CA139070
6 · The paper itself

Abstract

Combining the power of immunomagnetic assay and microfluidic microchip operations, we successfully detected rare CTCs from clinical blood samples. The microfluidic system is operated in a flip-flop mode, where a computer-controlled rotational holder with an array of microfluidic chips inverts the microchannels. We have demonstrated both theoretically and experimentally that the direction of red blood cell (RBC) sedimentation with regards to the magnetic force required for cell separation is important for capture efficiency, throughput, and purity. The flip-flop operation reduces the stagnation of RBCs and non-specific binding on the capture surface by alternating the direction of the magnetic field with respect to gravity. The developed immunomagnetic microchip-based screening system exhibits high capture rates (more than 90%) for SkBr3, PC3, and Colo205 cell lines in spiked screening experiments and successfully isolates CTCs from patient blood samples. The proposed motion controlled microchip-based immunomagnetic system shows great promise as a clinical tool for cancer diagnosis and prognosis.

Indexed as

MotionBlood SedimentationCell Line, TumorErythrocytesHumansImmunomagnetic SeparationMagnetic FieldsMicrofluidic Analytical TechniquesNanotechnologyNeoplasmsNeoplastic Cells, CirculatingViscosity

Identifiers

PMID23109037
PMCPMC3584207

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.