ArticleClinica chimica acta; international journal of clinical chemistry2013
The impact of antibody selection on the detection of cardiac troponin I.
Article in Clinica chimica acta; international journal of clinical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 24 citations in OpenAlex.
- Correlation between serum cardiac troponin I level and PDAI score in patients with pemphigus vulgaris.Archives of dermatological research · 2024Article
- Proteoforms and their expanding role in laboratory medicine.Practical laboratory medicine · 2022Review
- Development of Liposome-Based Immunoassay for the Detection of Cardiac Troponin I.Molecules (Basel, Switzerland) · 2021Article
- The role of antibody-based troponin detection in cardiovascular disease: A critical assessment.Journal of immunological methods · 2021Review
- Fluorescent Immunoassays for Detection and Quantification of Cardiac Troponin I: A Short Review.Molecules (Basel, Switzerland) · 2021Review
- Affinity-free enrichment and mass spectrometry analysis of the ovarian cancer biomarker CA125 (MUC16) from patient-derived ascites.The Analyst · 2021Article
- Development of a specific troponin I detection system with enhanced immune sensitivity using a single monoclonal antibody.Royal Society open science · 2020Article
- Analysis of cardiac troponin proteoforms by top-down mass spectrometry.Methods in enzymology · 2019Article
- Comprehensive Characterization of Swine Cardiac Troponin T Proteoforms by Top-Down Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2018Article
- Dissecting human skeletal muscle troponin proteoforms by top-down mass spectrometry.Journal of muscle research and cell motility · 2015Article
- Amino-functionalization of carbon nanotubes by using a factorial design: human cardiac troponin T immunosensing application.BioMed research international · 2014Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
backgroundCardiac troponin I (cTnI) is the current standard biomarker for diagnosing acute myocardial infarction and for risk-stratification of acute coronary syndromes in patients. However, it remains unclear how the epitope specificity of antibodies in immunoassays influences the detection of various modified forms of cTnI.
methodsFour mouse anti-human cTnI monoclonal antibodies targeting different regions of human cTnI were chosen for immunoaffinity purification of cTnI from human and swine cardiac tissue. High-resolution intact protein mass spectrometry was employed to assess the comparative performance of these four antibodies in detecting modified forms of cTnI.
resultsOur data revealed that antibody selection significantly impacts the relative protein yield of cTn from immunoaffinity purification. Remarkably, a single amino acid variation in cTnI (G->S) in the epitope region completely abolished the binding between monoclonal antibody 560 and swine cTnI in solution. Moreover, proteolytic degradation around the epitope region severely compromised the detection of proteolytic fragment forms of cTnI by monoclonal antibodies. In contrast, the phosphorylation status near the epitope region did not significantly affect the antibody recognition of cTnI.
conclusionCaution needs to be taken in the interpretation of the data produced by immuno-assays with monoclonal antibodies against various epitopes of cTnI.
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